Induction of neurite outgrowth through contactin and Nr-CAM by extracellular regions of glial receptor tyrosine phosphatase beta.
AuthorsSakurai T, Lustig M, Nativ M, Hemperly JJ, Schlessinger J, Peles E, Grumet M
JournalJ Cell Biol
PubMed ID9049255
'Receptor protein tyrosine phosphatase beta (RPTPbeta) is expressed as soluble and receptor forms with common extracellular regions consisting of a carbonic anhydrase domain (C), a fibronectin type III repeat (F), and a unique region called S. We showed previously that a recombinant Fc fusion protein with the C domain (beta ... More
Gap junctional communication in the early Xenopus embryo.
AuthorsLandesman Y, Goodenough DA, Paul DL
JournalJ Cell Biol
PubMed ID10953017
'In the Xenopus embryo, blastomeres are joined by gap junctions that allow the movement of small molecules between neighboring cells. Previous studies using Lucifer yellow (LY) have reported asymmetries in the patterns of junctional communication suggesting involvement in dorso-ventral patterning. To explore that relationship, we systematically compared the transfer of ... More
Conjugated avidin binds to mast cell granules.
AuthorsTharp MD, Seelig LL, Tigelaar RE, Bergstresser PR
JournalJ Histochem Cytochem
PubMed ID2578142
'The glycoprotein, avidin, conjugated either to the enzyme horseradish peroxidase, or to the fluorochrome dyes, fluorescein or rhodamine, identifies the granules of mast cells in both tissues and cell suspensions. In the absence of prior fixation, mast cells were not identified with conjugated avidin; however, granules released from these cells ... More
Rapid transport of large polymeric nanoparticles in fresh undiluted human mucus.
AuthorsLai SK, O'Hanlon DE, Harrold S, Man ST, Wang YY, Cone R, Hanes J
JournalProc Natl Acad Sci U S A
PubMed ID17244708
'Nanoparticles larger than the reported mesh-pore size range (10-200 nm) in mucus have been thought to be much too large to undergo rapid diffusional transport through mucus barriers. However, large nanoparticles are preferred for higher drug encapsulation efficiency and the ability to provide sustained delivery of a wider array of ... More
Crosslinkage and visualization of acetylcholine receptors on myotubes with biotinylated alpha-bungarotoxin and fluorescent avidin.
AuthorsAxelrod D
JournalProc Natl Acad Sci U S A
PubMed ID6933533
'A biotinylated derivative of alpha-bungarotoxin and tetramethylrhodamine-labeled avidin were employed to fluorescence label the acetylcholine receptors (AcChoR) on the surface of rat myotubes in primary culture. Because of the multivalency of both the biotinylated bungarotoxin and the avidin, this treatment extensivey crosslinks the AcChoR. AcChoR crosslinking immobilizes more than 90% ... More
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
AuthorsWood GS, Warnke R
JournalJ Histochem Cytochem
PubMed ID7028859
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed ... More
Multiple fluorescence in situ hybridization.
AuthorsNederlof PM, van der Flier S, Wiegant J, Raap AK, Tanke HJ, Ploem JS, van der Ploeg M
JournalCytometry
PubMed ID2307053
A method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue. This procedure is based on the labeling of (DNA) probes with more than one ... More
Visualization of a specific sequence on a single large DNA molecule using fluorescence microscopy based on a new DNA-stretching method.
AuthorsOana H, Ueda M, Washizu M
JournalBiochem Biophys Res Commun
PubMed ID10548504
A method for analyzing large DNA which makes it possible to obtain spatial information on the positions of specific sequences along a DNA molecule has been developed. Making use of the fact that large DNA molecules are stably elongated under an alternating-current field in a concentrated linear polymer solution, the ... More
Medullary thymic epithelial cells expressing Aire represent a unique lineage derived from cells expressing claudin.
AuthorsHamazaki Y, Fujita H, Kobayashi T, Choi Y, Scott HS, Matsumoto M, Minato N
JournalNat Immunol
PubMed ID17277780
The autoimmune regulator Aire is expressed in a small proportion of medullary thymic epithelial cells (mTECs) and is crucial in the induction of central T cell tolerance. The origin and development of Aire(+) mTECs, however, are not well understood. Here we demonstrate that the tight-junction components claudin-3 and claudin-4 (Cld3,4) ... More
Development of neuronal polarity: GAP-43 distinguishes axonal from dendritic growth cones.
AuthorsGoslin K, Schreyer DJ, Skene JH, Banker G
JournalNature
PubMed ID3059197
Outgrowth of distinct axonal and dendritic processes is essential for the development of the functional polarity of nerve cells. In cultures of neurons from the hippocampus, where the differential outgrowth of axons and dendrites is readily discernible, we have sought molecules that might underlie the distinct modes of elongation of ... More
Conjugated avidin identifies cutaneous rodent and human mast cells.
AuthorsBergstresser PR, Tigelaar RE, Tharp MD
JournalJ Invest Dermatol
PubMed ID6470526
Avidin conjugated to the fluorescent dyes rhodamine or fluorescein binds to mast cell granules in rodent and human skin. Sequential staining of tissue mast cells first with conjugated avidin, and then with a metachromatic stain demonstrated that both techniques identify the same mast cell granules. Specificity for mast cells was ... More
Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization.
Authorsdu Manoir S, Speicher MR, Joos S, Schröck E, Popp S, Döhner H, Kovacs G, Robert-Nicoud M, Lichter P, Cremer T
JournalHum Genet
PubMed ID8444465
Comparative genomic in situ hybridization (CGH) provides a new possibility for searching genomes for imbalanced genetic material. Labeled genomic test DNA, prepared from clinical or tumor specimens, is mixed with differently labeled control DNA prepared from cells with normal chromosome complements. The mixed probe is used for chromosomal in situ ... More
Tyrosine phosphorylation and acetylcholine receptor cluster formation in cultured Xenopus muscle cells.
AuthorsBaker LP, Peng HB
JournalJ Cell Biol
PubMed ID7678011
Aggregation of the nicotinic acetylcholine receptor (AChR) at sites of nerve-muscle contact is one of the earliest events to occur during the development of the neuromuscular junction. The stimulus presented to the muscle by nerve and the mechanisms underlying postsynaptic differentiation are not known. The purpose of this study was ... More