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Thermo Scientific™
EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which了解更多信息
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| 货号 | 数量 |
|---|---|
| A65394 | 500 Reactions |
| A65393 | 100 Reactions |
货号 A65394
价格（CNY)
16,051.00
Each
数量:
500 Reactions
价格（CNY)
16,051.00
Each
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Features of the EquiPhi29 DNA Amplification Kit include:
• Fast DNA amplification in less than two hours
• Sensitivity from 1 fg of input DNA
• Maximal yield of 17 µg amplified DNA
• Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
• Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
• All required components for DNA amplification in RCA or MDA-WGA reactions
The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.
仅供科研使用。不可用于诊断程序。
规格
产品规格Kit
最佳反应温度42°C
反应时间Up to 2 hours
运输条件Dry Ice
适用于（应用）RCA, MDA-WGA
形式Liquid
聚合酶EquiPhi29 DNA 聚合酶
产品类型DNA Amplification Kit
数量500 Reactions
研究类别Nucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
Unit SizeEach
内容与储存
• 5 x 100 μL EquiPhi29 DNA Polymerase (10 U/μL)
• 2 x 1 mL EquiPhi29 DNA Polymerase Reaction Buffer (10X)
• 0.25 mL DTT (0.1 M)
• 5 x 200 μL Exo-Resistant Random Primer (500 μM)
• 1 mL dNTP Mix (10 mM each)
• 5 x 100 μL Pyrophosphatase, inorganic (0.1 U/μL)
Store at -15 to -25°C.