Q: With the MagMAX Sequential DNA/RNA Kit, do you have a KingFisher purification system protocol to isolate nucleic acid from a sample input volume of lesser than 50 microliter of bone marrow?

We have not verified nucleic acid isolation from bone marrow using sample input volumes <50 µL. It may be possible to isolate nucleic acid from a sample input volume of <50 µL of bone marrow by bringing the volume up to 100 µL using phosphate buffered saline (PBS). Users are advised to conduct their own assessments and analytical validations in accordance with their laboratory guidelines. Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center .

Q: What type of blood collection tubes are compatible with the MagMAX Sequential DNA/RNA Kit?

Blood collection tubes containing K2 EDTA additive have been verified for whole blood and bone marrow samples. Users who utilize different blood collection devices or additives are advised to conduct their own assessments and analytical validations in accordance with their laboratory guidelines. Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center .

Q: With the MagMAX Sequential DNA/RNA Kit, what is the maximum sample input volume that can be used in the 96 deep-well format?

The maximum sample input volume that can be used with the 96 deep-well plate format is 150 µL. Sample input volumes greater than 150 µL, combined with reagents exceed the maximum well volume of 1 mL for a 96 deep-well plate. Sample input volumes ranging from 200-500 µL require the use of the 24 deep-well plate format. Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center .

Q: Can I use the MagMAX Sequential DNA/RNA Kit to isolate nucleic acid from cells or tissue samples?

We currently do not have workflows verified for sample types other than whole blood or bone marrow. Users are advised to conduct their own assessments and analytical validations in accordance with their laboratory guidelines. To isolate total RNA from cells and tissues, we recommend using the MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828) and to isolate DNA from cells and tissues, we recommend using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570). For sequential or single analyte isolation from FFPE tissue samples, we recommend using the MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881). Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center .

Q: In the MagMAX Sequential DNA/RNA Kit workflow, where should I add my samples for RNA processing?

The same Sample Plate is utilized for both DNA and RNA extractions. Initially, the Sample Plate is used for DNA extraction, and it should be retained for subsequent RNA extraction steps in the workflow. Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center .

Q: What is the maximum white bood cell (WBC) input for the MagMAX Sequential DNA/RNA Kit workflow?

A maximum of 2.5 million WBC/150 µL input (1.675 x 10E10/ L) or 7 million WBC/500 µL input (1.4 x 10E10/ L) can be used. Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center .

Question 1

Can I use the MagMAX Sequential DNA/RNA Kit to isolate small RNAs?

Accepted Answer

Yes, the MagMAX Sequential DNA/RNA Kit can be used to isolate total RNA, including small RNAs, down to 20 nucleotides.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 2

With the MagMAX Sequential DNA/RNA Kit, do you have a KingFisher purification system protocol to isolate nucleic acid from a sample input volume of  lesser than 100 microliter of whole blood?

Accepted Answer

We have not verified nucleic acid isolation from whole blood using sample input volumes <100 µL. It may be possible to isolate nucleic acid from a sample input volume of <100 µL of whole blood by bringing the volume up to 100 µL using phosphate buffered saline (PBS). Users are advised to conduct their own assessments and analytical validations in accordance with their laboratory guidelines.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 3

With the MagMAX Sequential DNA/RNA Kit, do you have a KingFisher purification system protocol to isolate nucleic acid from a sample input volume of lesser than 50 microliter of bone marrow?

Accepted Answer

We have not verified nucleic acid isolation from bone marrow using sample input volumes <50 µL. It may be possible to isolate nucleic acid from a sample input volume of <50 µL of bone marrow by bringing the volume up to 100 µL using phosphate buffered saline (PBS). Users are advised to conduct their own assessments and analytical validations in accordance with their laboratory guidelines.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 4

What type of blood collection tubes are compatible with the MagMAX Sequential DNA/RNA Kit?

Accepted Answer

Blood collection tubes containing K2 EDTA additive have been verified for whole blood and bone marrow samples. Users who utilize different blood collection devices or additives are advised to conduct their own assessments and analytical validations in accordance with their laboratory guidelines.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 5

With the MagMAX Sequential DNA/RNA Kit, what is the maximum sample input volume that can be used in the 96 deep-well format?

Accepted Answer

The maximum sample input volume that can be used with the 96 deep-well plate format is 150 µL. Sample input volumes greater than 150 µL, combined with reagents exceed the maximum well volume of 1 mL for a 96 deep-well plate. Sample input volumes ranging from 200-500 µL require the use of the 24 deep-well plate format.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 6

Can I use the MagMAX Sequential DNA/RNA Kit to isolate nucleic acid from cells or tissue samples?

Accepted Answer

We currently do not have workflows verified for sample types other than whole blood or bone marrow. Users are advised to conduct their own assessments and analytical validations in accordance with their laboratory guidelines. To isolate total RNA from cells and tissues, we recommend using the MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828) and to isolate DNA from cells and tissues, we recommend using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570). For sequential or single analyte isolation from FFPE tissue samples, we recommend using the MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881).

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 7

In the MagMAX Sequential DNA/RNA Kit workflow, where should I add my samples for RNA processing?

Accepted Answer

The same Sample Plate is utilized for both DNA and RNA extractions. Initially, the Sample Plate is used for DNA extraction, and it should be retained for subsequent RNA extraction steps in the workflow.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 8

What is the maximum white bood cell (WBC) input for the MagMAX Sequential DNA/RNA Kit workflow?

Accepted Answer

A maximum of 2.5 million WBC/150 µL input (1.675 x 10E10/ L) or 7 million WBC/500 µL input (1.4 x 10E10/ L) can be used.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

MagMAX™ Sequential RNA Elution Buffer, 32 mL - FAQs