DNA polyintercalating drugs: DNA binding of diacridine derivatives.
AuthorsLe Pecq JB, Le Bret M, Barbet J, Roques B
JournalProc Natl Acad Sci U S A
PubMed ID127172
'As a first step in the synthesis and the study of DNA polyintercalating drugs, dimers of acridines have been prepared. Their DNA binding properties have been studied. It has been determined that when the chain separating the two aromatic rings is longer than a criticål distance, bisintercalation is actually observed ... More
Deoxyribonucleic acid bifunctional intercalators: kinetic investigation of the binding of several acridine dimers to deoxyribonucleic acid.
AuthorsCapelle N, Barbet J, Dessen P, Blanquet S, Roques BP, Le Pecq JB
JournalBiochemistry
PubMed ID465475
Chromosome banding and identification. Fluorescence.
AuthorsSumner AT
JournalMethods Mol Biol
PubMed ID7518288
[DNA-intercalating compounds. Synthesis of acridine dimers]
AuthorsBarbet J, Roques BP, Le Pecq JB
JournalC R Acad Sci Hebd Seances Acad Sci D
PubMed ID813860
Several alpha, omega, di(2-methoxy-6-chloro-9-acridinyl) polyaza-alkanes have been prepared starting from 6, 9-dichloro-2-methoxy acridine. Some acridine dimers are able to bind to DNA with a very high affinity, both aromatic rings being intercalated. ... More
Review: ethidium fluorescence assays. Part 1. Physicochemical studies.
AuthorsMorgan AR, Lee JS, Pulleyblank DE, Murray NL, Evans DH
JournalNucleic Acids Res
PubMed ID41222
DNA and RNA can be assayed rapidly and very sensitively by exploiting the enhanced fluorescence of ethidium intercalated into duplex regions. By assaying at different pHs and introducing a heating/cooling cycle, a great many physicochemical aspects of DNA and RNA can be studied avoiding the use of radiolabels, and often ... More
Diacridines: bifunctional intercalators. I. Chemistry, physical chemistry and growth inhibitory properties.
AuthorsCanellakis ES, Shaw YH, Hanners WE, Schwartz RA
JournalBiochim Biophys Acta
PubMed ID1247545
The synthesis, as well as the rationale for synthesis of diacridines, double intercalators, as potential inhibitors of nucleic acid synthesis is presented. The syntheses of (9-acridyl)-putrescine and -spermine, and bis(-9-acridyl)-putrescine, -spermidine, -spermine diamines and of bis(6-chloro-2-methoxy-9-acridyl)-putrescine and -spermine diamines, all substituted on the terminal NH2 groups are described. In addition, ... More
Antagonism of immunostimulatory CpG-oligodeoxynucleotides by quinacrine, chloroquine, and structurally related compounds.
AuthorsMacfarlane DE, Manzel L
JournalJ Immunol
PubMed ID9570525
Phosphorothioate oligodeoxynucleotides containing CpG (CpG-ODN) activate immune responses. We report that quinacrine, chloroquine, and structurally related compounds completely inhibit the antiapoptotic effect of CpG-ODN on WEHI 231 murine B lymphoma cells and inhibit CpG-ODN-induced secretion of IL-6 by WEHI 231. They also inhibit IL-6 synthesis and thymidine uptake by human ... More