Regulation of cell movement is mediated by stretch-activated calcium channels.
AuthorsLee J, Ishihara A, Oxford G, Johnson B, Jacobson K
JournalNature
PubMed ID10432119
'Intracellular calcium regulates many of the molecular processes that are essential for cell movement. It is required for the production of actomyosin-based contractile forces, the regulation of the structure and dynamics of the actin cytoskeletons, and the formation and disassembly of cell-substratum adhesions. Calcium also serves as a second messenger ... More
Synthesis, photochemistry, and biological characterization of photolabile protecting groups for carboxylic acids and neurotransmitters.
AuthorsGee KR, Carpenter BK, Hess GP
JournalMethods Enzymol
PubMed ID9661143
Photoactivation of caged compounds in single living cells: an application to the study of cell locomotion.
AuthorsIshihara A, Gee K, Schwartz S, Jacobson K, Lee J
JournalBiotechniques
PubMed ID9266081
Caged compounds are molecules whose biological function is masked until UV light induces a photo-chemical reaction that converts the molecules into a biologically active state. These probes provide very powerful tools in cell biology research, since the activation can be precisely controlled temporally and spatially by limiting their exposure to ... More
Regulation of AMPA receptor lateral movements.
AuthorsBorgdorff AJ, Choquet D
JournalNature
PubMed ID12050666
An essential feature in the modulation of the efficacy of synaptic transmission is rapid changes in the number of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors at post-synaptic sites on neurons. Regulation of receptor endo- and exocytosis has been shown to be involved in this process. Whether regulated lateral diffusion of receptors ... More
Root hair growth in Arabidopsis thaliana is directed by calcium and an endogenous polarity.
AuthorsBibikova TN, Zhigilei A, Gilroy S
JournalPlanta
PubMed ID9421933
Tip growth of plant cells has been suggested to be regulated by a tip-focused gradient in cytosolic calcium concentration ([Ca2+]c). However, whether this gradient orients apical growth or follows the driving force for this process remains unknown. Using localized photoactivation of the caged calcium ionophore Br-A23187 we have been able ... More