Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen™

Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
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货号数量产品类型
A72127100 ReactionsLyo-ready RPA Kit
A721231600 μgLyo-ready T4 Gene 32 Protein
A72124120 μgLyo-ready T4 UvsX Protein
A72125120 μgLyo-ready T4 UvsY Protein
A721262000 μL反应缓冲液
A72128500 ReactionsLyo-ready RPA Kit
货号 A72127
价格（CNY)
2,559.00
Each
添加至购物车
数量:
100 Reactions
产品类型:
Lyo-ready RPA Kit
价格（CNY)
2,559.00
Each
添加至购物车
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
仅供科研使用。不可用于诊断程序。
规格
检测方法End point, real-time, lateral flow
最佳反应温度42°C
反应时间Up to 20 min.
逆转录酶SuperScript IV Reverse Transcriptase
运输条件Dry Ice
适用于（应用）RPA, RT-RPA
形式Liquid
聚合酶Lyo-ready Bst DNA Polymerase
产品类型Lyo-ready RPA Kit
数量100 Reactions
研究类别Nucleic Acid Amplification
Unit SizeEach
内容与储存

• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl2, 100 μL
• 10 mM dNTP Mix, 50 μL

Store at -25°C to -15°C.

常见问题解答 (FAQ)

Can I use PCR primers for target amplification by the Lyo-ready RPA Kit (Cat. No. A72127, A72128)?

PCR primers can be used with the Lyo-ready RPA Kit if they are at least 30 nt long. RPA primers can be designed using Primer3.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I lyophilize reagents from the Lyo-ready RPA Kit?

All RPA reagents (including Lyo-ready T4 UvsX Protein (Cat. No. A72124), Lyo-ready T4 UvsY Protein (Cat. No. A72125), Lyo-ready T4 Gene 32 Protein (Cat. No. A72123), Lyo-ready Bst DNA Polymerase (Cat. No. A56655), and RPA Reaction Buffer (2X) (Cat. No. A72126)) are lyo-ready, ensuring that enzymes and buffer formulations are glycerol-free. However, the formulations do not contain excipients needed for the lyophilization process.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

I noticed that my Lyo-ready RPA Kit is frozen after storage at -20 degrees C. Is it still good to use?

Lyo-ready RPA Kit and stand-alone reagents come in a storage buffer that does not contain glycerol. Product freezing doesn't affect its performance or stability. We do not recommend freeze-thawing Lyo-ready RPA Kit and stand-alone reagents for more than 20 cycles.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What is the temperature limit to perform an RPA reaction using a Lyo-ready RPA Kit (Cat. No. A72127, A72128)?

Lyo-ready RPA Kit can amplify DNA in temperatures ranging from 37 to 45 degrees C. To ensure the best performance, we recommend using the optimal reaction temperature of 42 degrees C.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I use my reverse transcriptase with a Lyo-ready RPA Kit (Cat. No. A72127, A72128) for RT-RPA?

We recommend using SuperScript IV Reverse Transcriptase (Cat. No. 18090010, 18090050, 18090200) for the best results in RT-RPA. Guidelines for use of SuperScript IV Reverse Transcriptase in RT-RPA application can be found in the Lyo-ready RPA Kit user guide. Other reverse transcriptases such as Maxima H Minus Reverse Transcriptase (Cat. No. EP0751) have also been tested and can be used in RT-RPA application. The list of Lyo-ready reverse transcriptases can be found here: thermofisher.com/lyo-ready.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

View all Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA) FAQs