The nucleolus exhibits an osmotically regulated gatekeeping activity that controls the spatial dynamics and functions of nucleolin.
AuthorsYang L, Reece JM, Cho J, Bortner CD, Shears SB,
JournalJ Biol Chem
PubMed ID18299322
'We demonstrate that physiologically relevant perturbations in the osmotic environment rheostatically regulate a gatekeeping function for the nucleolus that controls the spatial dynamics and functions of nucleolin. HeLa cells and U2-OS osteosarcoma cells were osmotically challenged with 100-200 mm sorbitol, and the intranuclear distribution of nucleolin was monitored by confocal ... More
Eight amino acids form the ATP recognition site of Na(+)/K(+)-ATPase.
AuthorsKubala M, Teisinger J, Ettrich R, Hofbauerová K, Kopecký V, Baumruk V, Krumscheid R, Plásek J, Schoner W, Amler E
JournalBiochemistry
PubMed ID12767226
'Point mutations of a part of the H(4)-H(5) loop (Leu(354)-Ile(604)) of Na(+)/K(+)-ATPase have been used to study the ATP and TNP-ATP binding affinities. Besides the previously reported amino acid residues Lys(480), Lys(501), Gly(502), and Cys(549), we have found four more amino acid residues, viz., Glu(446), Phe(475), Gln(482), and Phe(548), completing ... More
Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.
AuthorsDay JP, Kell DB, Griffith GW
JournalAppl Environ Microbiol
PubMed ID11772606
'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More