Eight amino acids form the ATP recognition site of Na(+)/K(+)-ATPase.
AuthorsKubala M, Teisinger J, Ettrich R, Hofbauerová K, Kopecký V, Baumruk V, Krumscheid R, Plásek J, Schoner W, Amler E
JournalBiochemistry
PubMed ID12767226
'Point mutations of a part of the H(4)-H(5) loop (Leu(354)-Ile(604)) of Na(+)/K(+)-ATPase have been used to study the ATP and TNP-ATP binding affinities. Besides the previously reported amino acid residues Lys(480), Lys(501), Gly(502), and Cys(549), we have found four more amino acid residues, viz., Glu(446), Phe(475), Gln(482), and Phe(548), completing ... More
Estimation of nuclear DNA content in plants using flow cytometry.
AuthorsDolezel J, Greilhuber J, Suda J
JournalNat Protoc
PubMed ID17853881
Flow cytometry (FCM) using DNA-selective fluorochromes is now the prevailing method for the measurement of nuclear DNA content in plants. Ease of sample preparation and high sample throughput make it generally better suited than other methods such as Feulgen densitometry to estimate genome size, level of generative polyploidy, nuclear replication ... More