Cationic gradient reversal and cytoskeleton-independent volume regulatory pathways define an early stage of apoptosis.
AuthorsBortner CD, Sifre MI, Cidlowski JA,
JournalJ Biol Chem
PubMed ID18187415
'Cell shrinkage, or apoptotic volume decrease (AVD), is a ubiquitous characteristic of programmed cell death that is independent of the death stimulus and occurs in all examples of apoptosis. Here we distinguished two specific stages of AVD based on cell size and a unique early reversal of intracellular ions that ... More
Eight amino acids form the ATP recognition site of Na(+)/K(+)-ATPase.
AuthorsKubala M, Teisinger J, Ettrich R, Hofbauerová K, Kopecký V, Baumruk V, Krumscheid R, Plásek J, Schoner W, Amler E
JournalBiochemistry
PubMed ID12767226
'Point mutations of a part of the H(4)-H(5) loop (Leu(354)-Ile(604)) of Na(+)/K(+)-ATPase have been used to study the ATP and TNP-ATP binding affinities. Besides the previously reported amino acid residues Lys(480), Lys(501), Gly(502), and Cys(549), we have found four more amino acid residues, viz., Glu(446), Phe(475), Gln(482), and Phe(548), completing ... More
Glutathione depletion and disruption of intracellular ionic homeostasis regulate lymphoid cell apoptosis.
Intracellular glutathione (GSH) depletion is an important hallmark of apoptosis. We have recently shown that GSH depletion by its extrusion regulates apoptosis independently of excessive reactive oxygen species accumulation. However, the mechanisms by which GSH depletion regulates apoptosis are still unclear. Because disruption of intracellular ionic homeostasis, associated with apoptotic ... More