Glutathione propagates oxidative stress triggered by myeloperoxidase in HL-60 cells. Evidence for glutathionyl radical-induced peroxidation of phospholipids and cytotoxicity.
AuthorsBorisenko GG, Martin I, Zhao Q, Amoscato AA, Tyurina YY, Kagan VE
JournalJ Biol Chem
PubMed ID15039448
'Glutathione acts as a universal scavenger of free radicals at the expense of the formation of the glutathionyl radicals (GS*). Here we demonstrated that GS* radicals specifically interact with a reporter molecule, paramagnetic and non-fluorescent 4-((9-acridinecarbonyl)-amino)-2,2,6,6-tetramethylpiperidine-1-oxyl (Ac-Tempo), and convert it into a non-paramagnetic fluorescent product, identified as 4-((9-acridinecarbonyl)amino)-2,2,6,6-tetramethylpiperidine (Ac-piperidine). Horseradish ... More
Reactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5.
AuthorsVeerman EC, Nazmi K, Van't Hof W, Bolscher JG, Den Hertog AL, Nieuw Amerongen AV
JournalBiochem J
PubMed ID15109304
'The mechanism of action of antimicrobial peptides is still a matter of debate. The formation of ROS (reactive oxygen species) has been suggested to be the crucial step in the fungicidal mechanism of a number of antimicrobial peptides, including histatin 5 and lactoferrin-derived peptides. In the present study we have ... More
A new method to study antioxidant capability: hydrogen transfer from phenols to a prefluorescent nitroxide.
AuthorsAliaga C, Aspée A, Scaiano JC
JournalOrg Lett
PubMed ID14572270
'[reaction: see text]. Rate constants for hydrogen abstraction from phenols by a prefluorescent-TEMPO probe are reported. The nitroxide is employed as a potential model of peroxyl radicals. The probe works by nitroxide suppression of the fluorescence of the chromophore. The fluorescence is restored when the nitroxide abstracts a hydrogen atom ... More
Comparison of fluorescence-based techniques for the quantification of particle-induced hydroxyl radicals.
AuthorsCohn CA, Simon SR, Schoonen MA,
JournalPart Fibre Toxicol
PubMed ID18307787
ABSTRACT: BACKGROUND: Reactive oxygen species including hydroxyl radicals can cause oxidative stress and mutations. Inhaled particulate matter can trigger formation of hydroxyl radicals, which have been implicated as one of the causes of particulate-induced lung disease. The extreme reactivity of hydroxyl radicals presents challenges to their detection and quantification. Here, ... More
A fluorescent probe for monitoring nitric oxide production using a novel detection concept.
AuthorsSoh N, Katayama Y, Maeda M
JournalAnalyst
PubMed ID11394293
A fluorescent probe using a novel 'spin exchange' concept was developed for monitoring nitric oxide (NO) production. The probe is composed of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) labeled with acridine and N-dithiocarboxysarcosine (DTCS)-Fe(II) complex. When the non-fluorescent acridine-TEMPO was incubated with DTCS-Fe(II) complex in buffer solution, the nitroxide radical in the acridine-TEMPO interacted ... More
Anti-D initially stimulates an Fc-dependent leukocyte oxidative burst and subsequently suppresses erythrophagocytosis via interleukin-1 receptor antagonist.
AuthorsCoopamah MD, Freedman J, Semple JW
JournalBlood
PubMed ID12829590
Previous results have demonstrated that anti-D therapy in children with chronic auto-immune thrombocytopenic purpura (AITP) induced a significant increase in several pro- and anti-inflammatory plasma cytokines within 2 hours of administration. To investigate the biologic basis of these early in vivo responses, we developed a flow cytometric assay to measure ... More