Verso cDNA Synthesis Kit, 100 reactions - FAQs

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

No, the Verso cDNA Syntheis Kits include RT Enhancer that, when included in the cDNA synthesis reaction, removes contaminating DNA eliminating the need for DNase I treatment.

Error rate is approximately 1 error per 15-27x 10^3 bases.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

The Verso can synthesize long cDNA strands, up to 11 kb, at a temperature range of 42°C to 57°C.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

The Verso cDNA synthesis Kit enables robust transcription of RNA to create a complete cDNA pool. The Verso system allows robust and sensitive reverse transcription through the combination of a high-affinity RT enzyme, a trusted RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.