MAXIscript™ T7 Transcription Kit, 30 reactions - FAQs

查看更多产品信息 MAXIscript™ T7 Transcription Kit - FAQs (AM1312, AM1314M, AM1314)

3 个常见问题解答

对于非同位素标记探针和放射性同位素标记探针您们分别提供哪些探针制备试剂盒？

我们提供两种非同位素探针标记试剂盒：

•The BrightStar Psoralen-Biotin Nonisotopic Labeling Kit (Cat. No. AM1480)
•MAXIscript Kits for in vitro transcription (Cat. Nos. AM1308 through AM1326)

我们提供3种放射性同位素探针标记试剂盒：

- 用于体外转录的MAXIscript试剂盒（货号AM1308至AM1326）
- KinaseMax5’末端标记试剂盒（货号AM1520）
- DECAprime II DNA标记试剂盒（货号AM1455）

What probe kits do you offer for nonisotopic probe labeling and radiolabeled probe labeling?

We offer two nonisotopic probe labeling kits:

- The BrightStar Psoralen-Biotin Nonisotopic Labeling Kit (Cat. No. AM1480)
- MAXIscript Kits for in vitro transcription (Cat. Nos. AM1308 through AM1326)

We offer three radiolabeled probe labeling kits:

- MAXIscript Kits for in vitro transcription (Cat. Nos. AM1308 through AM1326)
- KinaseMax 5' End-Labeling Kit (Cat. No. AM1520)
- DECAprime II DNA Labeling Kit (Cat. No. AM1455)

What kits should I use to isolate RNA from my sample?

The best isolation kit to use will depend greatly on your application, but, in general, the kits below produce RNA input compatible with the Ion Total RNA-Seq Kit v2 workflow:

Whole Transcriptome (polyA-selected or rRNA-depleted RNA is highly recommended, but total RNA is acceptable)
- Poly A-selected RNA
• Dynabeads mRNA DIRECT Micro Kit (Cat. No. 61021)
• MicroPoly(A)Purist Kit (Cat. No. AM1919)
- rRNA-depleted RNA
• RiboMinus Eukaryote System v2 (Cat. No. A15026)
• Low Input RiboMinus Eukaryote System v2 (Cat. No. A15027)
- Total RNA
• TRI Reagent
• TRizol
• RecoverAll MagMax

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.