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Invitrogen™
mMESSAGE mMACHINE™ T3 Transcription Kit
Obtain 10–50 times yield of conventional in vitro transcription reactions.
| Catalog Number | Product Type | No. of Reactions |
|---|---|---|
| AM1348 | T3 In Vitro Transcription kit | 25 Reactions |
Catalog number AM1348
Price (CNY)
8,029.00
Each
Product Type:
T3 In Vitro Transcription kit
No. of Reactions:
25 Reactions
Price (CNY)
8,029.00
Each
mMESSAGE mMACHINE high yield capped RNA transcription kits use patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions.
- High yields of capped RNA—up to 35 μg of RNA per reaction
- Extra convenience—cap analog-nucleotide mix provided
- Simplified reaction format
- Synthesized transcripts ideal for microinjection, in vitro translation, or transfection
- 10–50 times yield obtained with conventional in vitro transcription reactions
- Ratio of cap analog to GTP has been optimized to allow compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%)
- Contains a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
- Optimized for use with polymerases included in kit
- Contains 57 U of cap analog
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions25 Reactions
Product LineAmbion™, mMESSAGE mMACHINE™
Product TypeT3 In Vitro Transcription kit
PromoterT3
Quantity25 Reactions
Shipping ConditionDry Ice
Starting MaterialPCR Products, Linearized DNA plasmid
Yield20-30 μg
Final Product TypeSynthetic mRNA, mRNA
FormatKit
Sample TypemRNA, Synthetic mRNA
Unit SizeEach
Contents & Storage
T3 Enzyme Mix, 10X Reaction Buffer 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.