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查看更多产品信息 BrightStar® Psoralen-Biotin Nonisotopic Labeling Kit - FAQs (AM1480)
6 个常见问题解答
1. 样品纯度:
•样本中不能有游离核苷酸（例如，来自于PCR反应的游离核苷酸）。
• RNA样品中不能含有DNA，如转录模板。
•样本必须不含蛋白。
•核酸溶液应该不含酚或乙醇污染，pH值应当在2.5到10之间，而盐浓度应当少于20 mM。
2. 样本浓度应该在0.5–50 ng/μL之间。
它可以被用来标记单链反义RNA以用于Northern印迹，Southern印迹或原位杂交。该试剂盒还可以用于标记PCR产物，质粒，凝胶纯化的cDNA片段，以及用于Northern印迹、Southern印迹或原位杂交的寡核苷酸。当使用Ambion BrightStar BioDetect试剂盒进行非同位素检测和BrightStar-Plus正电性尼龙膜时，其灵敏度和32P放射性标记的探针以及酶促标记的生物素探针相当。
这些探针不会像放射性探针一样发生放射性衰变；所以你至少可以将它们保存一年。
1. Sample purity:
- There should be no free nucleotides in the preparation (e.g., from PCR).
- RNA samples must be free of DNA, e.g. a transcription template.
- Samples must be free of proteins.
- Nucleic acid solutions should be free of phenol or alcohol contaminants, the pH should be between 2.5 and 10, and the salt concentration should be less than 20 mM.
2. The sample concentration should be 0.5-50 ng/µL.
It can be used to label single-stranded antisense RNA for northerns, Southerns, or in situ hybridization. The same kit can also be used to label PCR products, plasmids, gel-purified cDNA inserts, and oligonucleotides for northern and Southern blotting and in situ hybridization. Sensitivity is comparable to that obtained with 32P-radiolabeled probes and enzymatically synthesized biotin-labeled probes when used with the BrightStar BioDetect Kit for nonisotopic detection and BrightStar-Plus Positively Charged Nylon Membranes.
These probes are not subject to radiolysis like radiolabeled probes; therefore, you can store them for at least one year.