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mirVana™ miRNA Isolation Kit, without phenol - FAQs

查看更多产品信息 mirVana™ miRNA Isolation Kit, without phenol - FAQs (AM1561)

5 个常见问题解答

纯化miRNA样本的不同方法有哪些？

沉淀miRNA：根据mirVANA miRNA Isolation试剂盒或mirVANA PARIS试剂盒的说明进行操作。
两次酚/胍(离液试剂)提取：使用酚/胍盐裂解组织，然后用酚提取，离心，取上清，并沉淀RNA。
玻璃纤维过滤：使用离液剂裂解组织，将RNA结合在玻璃纤维膜上，然后洗涤除去未结合的DNA/蛋白/盐/核苷酸，然后洗脱RNA。

What are different methods for cleaning up my miRNA sample?

- Precipitation of miRNA: Follow the instructions for the mirVANA miRNA Isolation Kit or the mirVANA PARIS RNA and Native Protein Purification Kit.
- Double phenol/guanidium (chaotropic reagent) extraction: Disrupt tissue with phenol/guanidine, then extract with phenol, centrifuge, remove the supernatant, and precipitate the RNA.
- Glass-fiber filter: Disrupt the tissue in a chaotropic agent, bind the RNA to a glass-fiber filter, then wash off unbound DNA/protein/salt/nucleotides and elute the RNA.

Can I order separately the Spin Cartridges and the Elution Tubes from the mirVana miRNA Isolation Kit, without phenol (Cat. No. AM1561)?

Yes, the Spin Cartridges and the Elution Tubes can be ordered separately. Please see the following links for the Spin Cartridges and Elution Tubes.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Do you offer a stand-alone phenol product that I can use with mirVana miRNA Isolation Kit, without phenol (Cat. No. AM1561)?

Yes, we offer Acid-Phenol: Chloroform, pH 4.5 (with IAA, 125:24:1), Cat. No. AM9720 or AM9722.
Note: We also offer mirVana miRNA Isolation Kit, with phenol (Cat. No. AM1560) that already comes with phenol.

What kit do you recommend to capture small RNA that is >17 nt in size?

We recommend using the mirVana miRNA Isolation Kit (Cat. No. AM1560, AM1561), both of which are designed to capture small RNAs, such as miRNAs, tRNAs, etc. Purification of RNAs as small as 20 nt has been obtained in-house using these kits. Note: Cat. No. AM1561 does not contain phenol, but is required for the procedure. Phenol can be purchased separately (Cat. No. AM9720).