MEGAscript™ RNAi 试剂盒
MEGAscript™ RNAi 试剂盒
Invitrogen™

MEGAscript™ RNAi 试剂盒

Ambion™ MEGAscript™ RNAi 试剂盒用于合成大量的 dsRNA 以用于非哺乳动物系统的 RNAi 实验。MEGAscript RNAi了解更多信息
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货号数量
AM162620 reactions
货号 AM1626
价格(CNY)
9,092.00
Each
添加至购物车
数量:
20 reactions
价格(CNY)
9,092.00
Each
添加至购物车
Ambion™ MEGAscript™ RNAi 试剂盒用于合成大量的 dsRNA 以用于非哺乳动物系统的 RNAi 实验。MEGAscript RNAi 试剂盒使用 Ambion 有专利的高产量转录技术,包括了用于转录、核酸酶酶切和纯化的试剂。该试剂盒包含可进行 20 次反应的足量试剂。

合成大量 dsRNA:
生成大量清洁、双链 RNA 的能力是 RNAi 实验成功的必要条件。MEGAscript RNAi 试剂盒可让 RNAi 研究人员在单一转录反应中就可以合成 50 至 100 µg 或者更多量的双链 RNA。其得率通常是常规转录反应产生的 RNA 量的 10–50 倍。PCR 或质粒来源的 DNA 均可用作转录反应的模板。正义链和反义链均可从 PCR 产生的 DNA 模板中合成,模板在其两端含有 T7 RNA 聚合酶启动子,从而无需单独的退火步骤。
仅供科研使用。不可用于诊断程序。
规格
反应次数20 reactions
产品线Ambion™、MEGAscript™
数量20 reactions
运输条件湿冰
控制类型转录对照
样品类型双链 RNA(长)
Unit SizeEach
内容与储存
T7 酶混合物、10X T7 反应缓冲液、ATP 溶液、CTP 溶液、GTP 溶液、UTP 溶液、10X 结合缓冲液、洗脱液、RNase、DNase I、10X 酶切缓冲液、2X 洗涤液、对照模板均储存在 –20°C 下,无核酸酶水可储存在任何温度。滤芯和收集管在室温下储存。

常见问题解答 (FAQ)

I am using the MEGAscript RNAi Kit for synthesizing dsRNA for RNAi. I ran my reaction product on a denaturing gel and got a smear. Can you offer some advice?

If the RNA appears degraded (e.g., smeared), remove residual RNase from the DNA template preparation before in vitro transcription. Do this by digesting the DNA prep with proteinase K (100-200 µg/mL) in the presence of 0.5% SDS for 30 min at 50°C, and follow this with phenol/chloroform extraction. The RNase Inhibitor that is present in the transcription reaction can only inactivate trace RNase contamination. Large amounts of RNase contamination will compromise the size and amount of transcription products.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the recommended shelf life of the MEGAscript RNAi Kit (Cat. No. AM1626)?

The MEGAscript RNAi Kit (Cat. No. AM1626) has no specific expiration date. However, for our products without specific expiration date, we guarantee best performance for one year effective from the date of receipt, when stored as recommended.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.