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View additional product information for Pre-miR™ miRNA Precursor Negative Control #1 - FAQs (AM17110)
12 product FAQs found
请访问miRBase（http://www.mirbase.org/）: The microRNA database（microRNA数据库）。
我们可以对Pre-miR和Anti-miR miRNA进行标记，但不提供标记的mirVana模拟物和抑制物。5’修饰包括Alexa Fluor 555, Alexa Fluor 647, FAM, 和Cy3。请点击此处（https://www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html）询价。
是的，请提交您的未发表的miRNA序列并/或点击此处（https://www.thermofisher.com/order/custom-genomic-products/tools/mirna/）订购定制修饰的miRNA。
Ambion Anti-miR抑制物和Ambion Pre-miR miRNA模拟物是我们的第一代抑制物和模拟物。Ambion Anti-miR抑制物是化学修饰的单链核酸，设计用于特异性结合并抑制内源microRNA (miRNA)分子。它们是可以使用转染或电转导入到细胞内的抑制物。针对miRBase version 15.0中的miRNA我们均设计了抑制物。
Ambion Pre-miR miRNA模拟物是化学修饰的短双链RNA分子，设计用于模拟内源成熟的miRNA。这些miRNA模拟物类似于siRNA，但不等同于siRNA，它们可以使用类似于siRNA的参数被转染或电转导入到细胞内。请注意，Pre-miR miRNA模拟物虽然叫Ambion Pre-miR miRNA Precursors，但并不是发夹结构，不能和miRNA前体相混淆。
mirVana miRNA模拟物和抑制物是我们的第二代模拟物和抑制物。mirVana miRNA模拟物是化学修饰的小双链RNA，它模拟内源成熟miRNA并通过上调miRNA活性使得研究miRNA功能成为可能。相比第一代模拟物它们的特异性有所提高，并且经HPLC纯化，可直接用于转染。
miRNA抑制物是化学修饰的小单链RNA分子，设计用于特异性结合并抑制内源miRNA分子并通过调低miRNA活性使得研究miRNA功能成为可能。相比第一代抑制物它们的性能有所提高。我们针对miRBase version 19.0中的miRNA均设计了抑制物。它们使用HPLC纯化，并可直接用于转染。想了解更多关于mirVana miRNA模拟物和抑制物的信息，请点击此视频（https://www.youtube.com/watch?feature=player_embedded&v=Q3MO1lmT0kw）。模拟物和抑制物均可以单独或作为文库订购。
它们既可用于体外也可用于体内实验，并且已验证可与Lipofectamine RNAiMAX转染试剂连用用于细胞水平的实验，和Invivofectamine 3.0试剂连用用于体内实验。这些寡核苷酸无毒并且在所测试的动物模型中未引发免疫反应。In vivo–ready mirVana miRNA模拟物和抑制物使用HPLC纯化并经过透析，可以直接用于体内实验。对于所有文库咨询，请电邮：rnailibraries@lifetech.com。
siRNA通过序列特异的方式介导目标mRNA分子的降解，而miRNA抑制翻译而不剪切mRNA。
miRNA (microRNA)是植物和动物基因组中天然产生的短链、高度保守的小非编码RNA分子。miRNA是单链的，长18-25nt并且可以调控转录后mRNA水平，它们通常结合互补mRNA序列的3’非翻译区(3’-UTR)，导致翻译抑制和基因沉默。研究表明数以千计的人类蛋白编码基因被miRNA所调控，说明miRNA是很多重要生物进程的“重要调控者”。
Please visit miRBase: The microRNA database (http://www.mirbase.org/).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
We can label our Pre-miR and Anti-miR miRNA, but do not offer labeled mirVana Mimics and inhibitors. 5' modifications include Alexa Fluor 555, Alexa Fluor 647, FAM, and Cy3. Request a quote here (http://www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Yes, submit your unpublished miRNA sequences and/or order custom modified miRNA here (http://www.thermofisher.com/order/custom-genomic-products/tools/mirna/).
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors are our first generation mimic and inhibitors. Invitrogen Anti-miR miRNA Inhibitors are chemically modified, single stranded nucleic acids designed to specifically bind and inhibit endogenous microRNA (miRNA) molecules. These are ready to use inhibitors that can be introduced into cells using transfection or electroporation. Pre-designed inhibitors have been created for all miRNAs listed in miRBase version 15.0.
Invitrogen Pre-miR miRNA Precursors are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs. These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Please note, Pre-miR miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
mirVana miRNA mimics and inhibitors are our second generation of mimics and inhibitors. mirVana miRNA mimics are small, chemically double-stranded RNAs that mimic endogenous mature miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity. They have enhanced specificity over the first generation mimics, are delivered as HPLC purified, and ready to transfect.
miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity. They have enhanced potency over our first generation inhibitors. Pre-designed inhibitors were created for all miRNAs listed in miRBase version 19.0. They come delivered as HPLC purified, and ready to transfect. Learn more about mirVana miRNA mimics and inhibitors through this video (https://www.youtube.com/watch?feature=player_embedded&v=Q3MO1lmT0kw). Both mimics and inhibitors are available individually or as libraries.
They are compatible with both in vitro and in vivo applications, and have been validated with Lipofectamine RNAiMAX Transfection Reagent for use in cell-based systems, and with Invivofectamine 3.0 Transfection Reagent for in vivo delivery. These oligonucleotides are nontoxic and do not induce an immune response in the animal models tested. In vivo-ready mirVana miRNA mimics and inhibitors have been purified by HPLC and dialysis and are ready for immediate in vivo use. For all library inquiries, please email: rnailibraries@lifetech.com.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
siRNA mediates degradation of a target mRNA molecule in a sequence-specific manner, while miRNA suppresses translation without cleaving mRNA.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
miRNA (microRNAs) are short, highly conserved small noncoding RNA molecules naturally occurring in the genomes of plants and animals. miRNAs are single stranded, 18-25 nucleotides long and regulate posttranscriptional mRNA expression, typically by binding to the 3' untranslated region (3'-UTR) of the complementary mRNA sequence, resulting in translational repression and gene silencing. Studies have shown that thousands of human protein-coding genes are regulated by miRNAs, indicating that miRNAs are “master regulators” of many important biological processes.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.