Q: I need a recommendation for a MagMAX kit for automated viral RNA isolation from Animal Health samples such as serum, nasal fluid, and swab samples. What would you recommend?

Cat. No. AM1836 is a good choice if you want to process 50 µL of samples. If you want to maximize the input amount and use more than that (up to 300 µL) we recommend using the MagMAX RNA/DNA Pathogen kit (Cat. No. 4462359). The buffers and reagents included in these MagMAX kits are identical, but the volumes are larger for the MagMAX Pathogen RNA/DNA kit allowing for more sample input.

Question 1

我需要一个MagMAX试剂盒用于从动物健康样本(如血清、鼻液和拭子样本)中自动分离病毒RNA。你有什么推荐的吗?

Accepted Answer

< p >如果您想加工50微升样品，货号AM1836是一个不错的选择。如果你想最大化样品输入量（多至300微升），我们建议使用MagMAX RNA/DNA病原体试剂盒(货号4462359)。这些MagMAX试剂盒中包含的缓冲液和试剂是相同的，但是MagMAX病原体RNA/DNA试剂盒的容量更大，允许更大的样本体积。< / p >

Question 2

MagMax试剂盒是否与RNAlater试剂处理的样品兼容?

Accepted Answer

只有MagMax96用于微阵列总RNA分离试剂盒(货号AM1839)是兼容的，因为它使用Trizol试剂，大部分来自RNAlater试剂的残留的盐在裂解步骤中被去除，并与上层的RNA水相分开。对于其他的MagMax试剂盒，需要进行优化。高盐携带并不是MagMax RNA分离步骤的最佳选择。

Question 3

我可以编辑出厂自带的KingFisher Flex实验方案吗？

Accepted Answer

不能，出厂实验方案不能编辑，但是您可以使用“另存为”功能对其重命名，然后将它作为一个新的版本来编辑。

Question 4

我可以在KingFisher Flex仪器上使用其它厂家生产的磁珠吗？

Accepted Answer

对于核酸提取，我们建议使用我们的MagMAX试剂盒和MagJET试剂盒。其它大小在0.5到10 µm间的磁珠应该也可以使用。但是，您需要自己用Thermo Scientific BindIt软件编写您自己的KingFisher实验方案，并自己验证/优化这一实验方案。

Question 5

我想使用KingFisher Flex仪器从大体积的液体样本中提取核酸。我可以使用比实验方案中建议的体积更多的样本吗？

Accepted Answer

可以，可将样品分装。在进行下一步之前，设置程序使得磁头可以从多个孔内收集样本。确保不要过量加样。

Question 6

使用KingFisher Flex仪器时我可以扩大我的反应体积吗？

Accepted Answer

可以，反应体积可以扩大，并且所有其它体积（例如洗涤体积）需要以相同倍数扩大。除非板的容积允许，我们强烈建议反应体积扩大不应超过2倍。

Question 7

所有Applied Biosystems MagMAX试剂盒都能用于Thermo Scientific KingFisher Flex 磁珠处理仪吗？

Accepted Answer

是的，所有MagMAX试剂盒都能用于KingFisher Flex 磁珠处理仪。

Question 8

I need a recommendation for a MagMAX kit for automated viral RNA isolation from Animal Health samples such as serum, nasal fluid, and swab samples. What would you recommend?

Accepted Answer

Cat. No. AM1836 is a good choice if you want to process 50 &micro;L of samples. If you want to maximize the input amount and use more than that (up to 300 &micro;L) we recommend using the MagMAX RNA/DNA Pathogen kit (Cat. No. 4462359). The buffers and reagents included in these MagMAX kits are identical, but the volumes are larger for the MagMAX Pathogen RNA/DNA kit allowing for more sample input.

Question 9

Is the the direct lysis approach to DNA/RNA analysis applicable for extraction of viral RNA?

Accepted Answer

Some customers have isolated RNA from viruses.  Unfortunately, the protein coat could cause problems.  Another option would be to use the MagMax Viral Isolation Kit.

Question 10

Are MagMax kits compatible with RNAlater reagent-treated samples?

Accepted Answer

Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed. High salt carry over is not optimal for MagMax RNA isolation steps.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Question 11

Can I edit the manufactured KingFisher Flex protocols?

Accepted Answer

No, manufactured protocols are locked for editing, but they can be renamed using the &#145;save as' function and edited as a new version.

Question 12

Can I use magnetic beads from other vendors with the KingFisher Flex instrument?

Accepted Answer

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 13

I am working with liquid samples with high volume and want to extract nucleic acid from them using the KingFisher Flex instrument. Can I use more volume than the protocol advises?

Accepted Answer

Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Question 14

Can I scale up my reaction volume with the KingFisher Flex instrument?

Accepted Answer

Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.

Question 15

Are all of the Applied Biosystems MagMAX kits validated for the KingFisher Flex Magnetic Particle Processor?

Accepted Answer

Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.

MagMAX™-96 Viral RNA Isolation Kit, 96 preps - FAQs