MEGAclear™ Transcription Clean-Up Kit - FAQs

以下方法可去除DNase：酸性苯酚：氯仿抽提、氯化锂沉淀、EDTA和热灭活，或使用我们的MegaClear 转录回收试剂盒（货号AM1908）。
此外，我们的Ambion TURBO DNA-free试剂盒（货号AM1907）包含DNase灭活试剂，可用于去除DNase以及镁和钙等二价阳离子，这些物质在RNA随样品一起加热时可催化RNA降解。

DNase can be removed by the following methods: acid phenol:choloform extraction, lithium chloride precipitation, EDTA, and heat inactivation, or using our MegaClear Transcription Clean-Up Kit (Cat. No. AM1908).

Alternatively, our Invitrogen TURBO DNA-free Kit (Cat. No. AM1907) is supplied with a DNase inactivation reagent that can be used to remove the DNase, as well as divalent cations such as magnesium and calcium, which can catalyze RNA degradation when RNA is heated with the sample.

The binding capacity of the 10051G filter columns is 500µg of RNA

Yes, we do offer the Spin Cartridges (Cat. No. AM10051G) as a stand-alone product, and they would be compatible with the MEGAclear Transcription Clean-Up Kit (Cat. No. AM1908).

Yes, the Elution Tubes (2.0 mL) (Cat. No. AM12480) are the same that are used in the MEGAclear Transcription Clean-Up Kit (Cat. No. AM1908).

It is 0.1 mM EDTA (pH 8.0) in nuclease-free water.