• 50 mL 无核酸酶水;在室温下储存 • 50 mL 洗脱溶液;在室温下储存 • 2个过滤板;在室温下储存 • 2个收集板;在室温下储存 • 2个胶板封口机;在室温下储存 • 2个板盖;在室温下储存 • 50 mL 裂解/结合溶液;在 4°C 下储存 • 175 mL 洗涤溶液(浓缩液);在 4°C 下储存 • 53 mL 再结合混合物;在 4°C 下储存 • 12 mL DNase I 缓冲液;在 -20°C 下储存 • 1.25 mL DNase I;在 -20°C 下储存
常见问题解答 (FAQ)
不同的RNAqueous试剂盒有何区别?
RNaqueous试剂盒是基于快速的过滤法RNA分离系统,不需要苯酚、氯仿或其他有毒有机试剂。RNAqueous总RNA分离试剂盒专用于10–75 mg的组织或10^6-10^7的细胞样品,而经过优化的RNAqueous-Midi总RNA分离试剂盒是用来从多至0.5g组织或10^9个细胞样品中提取总RNA的试剂盒。RNAqueous-Micro微量总RNA提取试剂盒可用于从微量样本中纯化总RNA,如10–500,000个培养细胞、少至10个显微切割细胞或最多10 mg的组织。RNAqueous-96总RNA分离试剂盒利用96孔板格式,对100至2 x 10^6个细胞或0.1至1.5 mg组织进行高通量RNA分离。RNAqueous-4PCR总RNA分离试剂盒专用于从少量组织样品(0.5–75 mg)以及少量至中量培养细胞样品(约100–10^7个细胞)中分离RNA。
What are the differences in the RNAqueous kits you offer?
RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).
Can you please recommend the best manifold to use with the RNAqueous-96 Total RNA Isolation Kit (Cat. No. AM1920)?
The EveryPrep Universal Vacuum Manifold (Cat. No. K211101) is compatible with the RNAqueous-96 Total RNA Isolation Kit.