RiboPure™ RNA Purification Kit, yeast - FAQs

查看更多产品信息 RiboPure™ RNA Purification Kit, yeast - FAQs (AM1926)

4 个常见问题解答

RiboPure RNA纯化试剂盒的柱分离临界尺寸是多少？

柱分离临界尺寸为200 nt。RiboPure试剂盒不适用于回收miRNA。我们推荐使用mirVana试剂盒、TRIzol Plus RNA纯化试剂盒或PureLink RNA微量提取试剂盒分离miRNA。

PureLink RNA小量提取试剂盒和RiboPure RNA纯化试剂盒在技术方面有何差异？

PureLink RNA小量提取试剂盒提供快速的总RNA柱纯化，无需有机溶解（苯酚/氯仿）。单次提取可获得多达1,000 µg的纯化RNA。RiboPure RNA纯化试剂盒结合了苯酚/硫氰酸胍溶液和玻璃纤维过滤纯化法。

What is the column cut-off size for the RiboPure RNA Purification Kits?

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.