Stability of cord blood RNA measured by house keeping transcripts: relevance for large-scale studies of childhood leukaemia.
AuthorsOlsen M, Madsen HO, Hjalgrim H, Ford A, Schmiegelow K
JournalLeukemia
PubMed ID17051240
Comparison of the MagNA pure LC automated system and the RiboPure-Blood RNA manual method for RNA extraction from multiple myeloma bone marrow samples conserved in an RNA stabilizer.
'A total of 62 frozen bone marrow specimens conserved in RNA later (Ambion) were processed using two different extraction methods, the MagNA Pure LC system (MAG; Roche) and the manual RiboPure-Blood RNA method (RIBO; Ambion); Beta glucoronidase RNA (GUS) was amplified by LightCycler PCR to evaluate the quality of both ... More
Expression profile of MicroRNAs in young stroke patients.
AuthorsTan KS, Armugam A, Sepramaniam S, Lim KY, Setyowati KD, Wang CW, Jeyaseelan K
JournalPLoS One
PubMed ID19888324
The methods currently available for diagnosis and prognosis of cerebral ischaemia still require further improvements. Micro-RNAs (small non-coding RNAs) have been recently reported as useful biomarkers in diseases such as cancer and diabetes. We therefore carried out microRNA (miRNA) profiling from peripheral blood to detect and identify characteristic patterns in ... More
MicroRNA expression in the blood and brain of rats subjected to transient focal ischemia by middle cerebral artery occlusion.
AuthorsJeyaseelan K, Lim KY, Armugam A
JournalStroke
PubMed ID18258830
Several hundred small RNAs called microRNAs (miRNAs) have been identified and characterized from various organisms, including humans. In humans, some of these miRNAs have been found to regulate (patho)physiologic conditions such as tumor progression/regression, cholesterol and glucose homeostasis, etc. In this report, we present data on the miRNAs expressed under ... More
Nonsense mutations in hERG cause a decrease in mutant mRNA transcripts by nonsense-mediated mRNA decay in human long-QT syndrome.
AuthorsGong Q, Zhang L, Vincent GM, Horne BD, Zhou Z
JournalCirculation
PubMed ID17576861
Long-QT syndrome type 2 (LQT2) is caused by mutations in the human ether-a-go-go-related gene (hERG). More than 30% of the LQT2 mutations result in premature termination codons. Degradation of premature termination codon-containing mRNA transcripts by nonsense-mediated mRNA decay is increasingly recognized as a mechanism for reducing mRNA levels in a ... More