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RiboPure™ RNA Purification Kit, blood - FAQs

查看更多产品信息 RiboPure™ RNA Purification Kit, blood - FAQs (AM1928)

11 个常见问题解答

RiboPure血液纯化试剂盒（货号AM1928）与Mouse RiboPure血液RNA分离试剂盒（货号AM1951）有何区别？

前者（货号AM1928）用于从人类血液中分离RNA，后者（货号AM1951）用于从小鼠和大鼠血液中分离RNA。两种试剂盒就有相同的纯化柱，但洗涤缓冲液1、洗涤缓冲液2/3和醋酸钠溶液不同。我们不推荐将小鼠试剂盒用于人类血液样品。小鼠试剂盒可适应和人类血液不同的小鼠/大鼠血液粘稠度，以及能获得较高的小鼠和大鼠血液RNA产量。根据小鼠试剂盒使用手册中的不同实验方案，可回收含有小RNA的总RNA或不含小RNA的总RNA。使用RiboPure 血液纯化试剂盒使用手册中的标准实验方案时，可分离不含小RNA的总RNA，而使用另一种替代方案时，可分离含小RNA的总RNA。此外，若只想得到小RNA，则应先采用上述替代方案得到含小RNA的总RNA，然后遵循mirVana miRNA分离试剂盒使用手册中的IVA部分（货号AM1560）。

RiboPure血液纯化试剂盒（货号AM1928）中洗脱液的成分是什么？

成分是0.1 mM EDTA溶于无核酸酶的水。

RiboPure RNA纯化试剂盒的柱分离临界尺寸是多少？

柱分离临界尺寸为200 nt。RiboPure试剂盒不适用于回收miRNA。我们推荐使用mirVana试剂盒、TRIzol Plus RNA纯化试剂盒或PureLink RNA微量提取试剂盒分离miRNA。

PureLink RNA小量提取试剂盒和RiboPure RNA纯化试剂盒在技术方面有何差异？

PureLink RNA小量提取试剂盒提供快速的总RNA柱纯化，无需有机溶解（苯酚/氯仿）。单次提取可获得多达1,000 µg的纯化RNA。RiboPure RNA纯化试剂盒结合了苯酚/硫氰酸胍溶液和玻璃纤维过滤纯化法。

你们是否有从血液样品中分离RNA的技巧？

请访问我们的网站（https://www.thermofisher.cn/cn/en/home/references/ambion-tech-support/rna-isolation/general-articles/tips-for-working-with-blood-samples.html），查看处理血液样品的技巧。

What is the difference between the RiboPure Blood Purification Kit (Cat. No. AM1928) and Mouse RiboPure Blood RNA Isolation kit (Cat. No. AM1951)?

The former (Cat. No. AM1928) is for isolation of RNA from human blood and the latter (Cat. No. AM1951) is for isolation of RNA from mouse and rat blood. Both kits have the same columns but differ in protocol and the wash 1 buffer, wash 2/3 buffer, and sodium acetate solutions. We do not recommend using the mouse kit for human blood samples. The mouse kit accommodates the difference in viscosity of mouse/rat vs. human blood and also the higher mouse and rat blood RNA yield. The mouse kit allows recovery of either total RNA containing small RNAs or total RNA depleted of small RNAs based on which protocol in the manual was followed. With the RiboPure Blood Purification Kit, using the standard protocol in the manual, you can isolate total RNA depleted of small RNAs, but with an alternate protocol, you can isolate total RNA containing small RNAs. Alternatively, if you need small RNAs alone, then you should follow the above alternate protocol for isolation of total RNAs containing small RNAs, and then follow Section IVA in the mirVana miRNA Isolation Kit manual (Cat. No AM1560).

What is the composition of the elution solution in the RiboPure-Blood Purification Kit (Cat. No. AM1928)?

Its composition is 0.1 mM EDTA in nuclease-free water.

What is the column cut-off size for the RiboPure RNA Purification Kits?

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.

Do you have any tips for RNA isolation when working with blood samples?

Please visit our website for tips for working with blood samples.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Can I use the RiboPure RNA Purification Kit, blood (Cat. No. AM1928) to extract RNA from blood samples?

For RNA purification from blood samples, in RNAlater, the RiboPure RNA Purification Kit, blood is recommended.
Please note the ratio of blood to RNAlater should be 300-500 µL blood for 1.3 mL of RNAlater.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.