Mouse RiboPure™-Blood RNA Isolation Kit with Manual - FAQs

查看更多产品信息 Mouse RiboPure™-Blood RNA Isolation Kit with Manual - FAQs (AM1951M)

5 个常见问题解答

RiboPure RNA纯化试剂盒的柱分离临界尺寸是多少?

柱分离临界尺寸为200 nt。RiboPure试剂盒不适用于回收miRNA。我们推荐使用mirVana试剂盒、TRIzol Plus RNA纯化试剂盒或PureLink RNA微量提取试剂盒分离miRNA。

PureLink RNA小量提取试剂盒和RiboPure RNA纯化试剂盒在技术方面有何差异?

PureLink RNA小量提取试剂盒提供快速的总RNA柱纯化,无需有机溶解(苯酚/氯仿)。单次提取可获得多达1,000 µg的纯化RNA。RiboPure RNA纯化试剂盒结合了苯酚/硫氰酸胍溶液和玻璃纤维过滤纯化法。

What is the column cut-off size for the RiboPure RNA Purification Kits?

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.

Do Tempus Blood RNA Tubes (Cat. No. 4342792) stabilize RNA similarly to RNAlater Stabilization Solution (Cat. No. AM7021)?

Both Tempus Blood RNA Tubes and RNAlater Stabilization Solution employ the same effective stabilization of mRNA expression profiles, and eliminate the need to isolate the RNA immediately after sample collection. However, there are some key differences:
- Components in each stabilization solution are different. The exact components and differences are proprietary.
- The RNAlater Stabilization Solution preserves RNA from degradation without lysing blood cells.
- Samples in RNAlater Stabilization Solution can be safely stored for up to 1 week at 25 degrees C, or up to 1 month at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.