Ambion™ Recombinant DNase I (rDNase I) - FAQs

查看更多产品信息 Ambion™ Recombinant DNase I (rDNase I) - FAQs (AM2235)

4 个常见问题解答

DNase I需要哪些辅助因子？DNase I会消化ssDNA吗？

镁和钙是DNase I所需的辅助因子。DNase I确能消化ssDNA。

What cofactors are required for DNase I? Does DNase I digest ssDNA?

Magnesium and calcium are required cofactors for DNase I. DNase I does digest ssDNA.

How can I inactivate DNase I?

Add of 1 µL of 25 mM EDTA solution to the reaction mixture in 10 µL reaction with 1 unit DNase I, Amplification Grade (or 1:1 molar ratio of Mg++ ions:EDTA) to chelate the Mg++ ions in the DNase I buffer. Heat for 10 min at 65 degrees C.

Note: It is vital that the EDTA be at least at 2 mM prior to heat-inactivation to avoid Mg-dependent RNA hydrolysis.

DNA-free and Turbo-free versions of DNase I can be inactivated with included DNase Inactivation Reagent.

Are your DNase I products RNase-free?

Most of our DNase I products are guaranteed free of RNase activity. However, please note that Cat. No. 18047-019 is not tested for RNAse and is recommended primarily for protein applications. The other products are suitable for removing DNA from both RNA and protein preparations, for nick translating DNA, and for generating random fragments of DNA. For more demanding RT-PCR applications, we recommend using DNAse I, Amplification Grade.