Recombinant Proteinase K Solution (20 mg/mL), 1.25 mL - FAQs

查看更多产品信息 Recombinant Proteinase K Solution (20 mg/mL) - FAQs (AM2548, AM2546)

2 个常见问题解答

How do I isolate DNA from single bacterial colonies before PCR?

Scrape a single colony from the surface of an agar plate and transfer to 12 µL of SCL solution [10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 50 µg/mL proteinase K] and incubate for 15 min at 55 degrees C. Inactivate proteinase K for 15 min at 80 degrees C and add 20 µL of deionized water. Centrifuge at 12,000 x g for 3 min. Transfer the supernatant to a new tube.

I received Recombinant Proteinase K Solution (Cat. No. AM2546, AM2548) on Friday and it sat over the weekend and thawed. Is it still usable?

No, we do not recommend using it.