在siRNA实验中包含阳性和阴性对照有多关键？
阳性对照siRNA例如Silencer Select GAPDH siRNA将证明转染的有效性并有助于优化转染条件。阴性对照siRNA对于设定基因表达的基线是必要的。
我的Silencer或Silencer Select siRNA实验应该使用什么阳性和阴性对照？
对于Silencer和Silencer Select siRNA我们均提供多个阴性和阳性对照。
Silencer siRNA阳性对照有：
- Silencer Firefly Luciferase (GL2 + GL3) siRNA（货号AM4629, 5nmol）
- Silencer GAPDH siRNA（人）（货号AM4605, 5nmol; AM4633, 40 nmol）
- Silencer GAPDH siRNA (人，小鼠，大鼠) (货号AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
- Silencer GFP (eGFP) siRNA （货号AM4626, 5 nmol）
- Silencer KIF11 (Eg5) siRNA (人，小鼠，大鼠) (货号AM4639, 5 nmol）
如果使用荧光标记的Silencer siRNA，我们有：
- Silencer Cy-3标记GAPDH siRNA (人，小鼠，大鼠) (货号AM4649, 5 nmol)
- Silencer FAM-标记GAPDH siRNA(人，小鼠，大鼠) (货号AM4650, 5 nmol)
如果使用体内级别的Silencer siRNA，可以选：
- Silencer GAPDH阳性对照siRNA，in vivo ready（货号4404025, 250 nmol）
Silencer siRNA阴性对照有：
- Silencer阴性对照1号 siRNA（货号AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol）
- Silencer阴性对照2号siRNA（货号AM4613, 5 nmol; AM4637, 30 nmol）
- Silencer阴性对照3号 siRNA（货号AM4615, 5 nmol）
- Silencer阴性对照4号 siRNA（货号AM4641, 5 nmol）
- Silencer阴性对照5号 siRNA（货号AM4642, 5 nmol）
如果使用荧光标记的Silencer siRNA，我们有：
- SilencerCy 3-标记阴性对照1号siRNA（货号AM4621, 5 nmol）
- Silencer FAM-标记阴性对照1号 siRNA（货号AM4620, 5 nmol）
如果使用体内级别的Silencer siRNA，可以选：
- Silencer 阴性对照 1号 siRNA（货号4404021）
Silencer Select siRNA阳性对照有：
- Silencer Select GAPDH阳性对照siRNA（货号4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol）
- Silencer Select MALAT1阳性对照siRNA，靶向非编码siRNA (货号4455877, 5 nmol)
如果使用体内级别的Silencer Select siRNA，可以选：
- Silencer Select GAPDH in vivo（货号44404024）
Silencer Select siRNA阴性对照有：
- Silencer Select阴性对照1号 siRNA (货号4390843, 5 nmol; 4390844, 40 nmol)
- Silencer Select阴性对照2号 siRNA (货号4390846, 5 nmol; 4390847, 40 nmol)
如果使用体内级别的Silencer Select siRNA，可以选：
- Silencer Select阴性对照1号 siRNA, 体内级别 (货号4404020, 250nmol)
How critical is it to include positive and negative controls in siRNA experiments?
A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
What are the positive and negative controls I should use with my Silencer or Silencer Select siRNA experiment?
We offer several negative and positive controls for both Silencer and Silencer Select siRNA.
For Silencer siRNA positive control:
- Silencer Firefly Luciferase (GL2 + GL3) siRNA (Cat. No. AM4629, 5nmol)
- Silencer GAPDH siRNA (human) (Cat. No AM4605, 5nmol; AM4633, 40 nmol)
- Silencer GAPDH siRNA (human, mouse, rat) (Cat. No. AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
- Silencer GFP (eGFP) siRNA (Cat. No. AM4626, 5 nmol)
- Silencer KIF11 (Eg5) siRNA (human, mouse, rat) (Cat. No. AM4639, 5 nmol)
If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy-3 labeled GAPDH siRNA (human, mouse, rat) (Cat. No. AM4649, 5 nmol)
- Silencer FAM-labeled GAPDH siRNA (human, mouse, rat) (Cat. NO. AM4650, 5 nmol)
If working with Silencer siRNA in vivo ready:
- Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)
For Silencer siRNA negative controls:
- Silencer Negative Control No. 1 siRNA (Cat. Nos. AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
- Silencer Negative Control No. 2 siRNA (Cat. Nos. AM4613, 5 nmol; AM4637, 30 nmol)
- Silencer Negative Control No. 3 siRNA (Cat. No. AM4615, 5 nmol)
- Silencer Negative Control No. 4 siRNA (Cat. No. AM4641, 5 nmol)
- Silencer Negative Control No. 5 siRNA (Cat. No. AM4642, 5 nmol)
If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No AM4621, 5 nmol)
- Silencer FAM-labeled Negative Control No. 1 siRNA (Cat. No. AM4620, 5 nmol)
If working with Silencer siRNA in vivo ready:
- Silencer in vivo ready (Cat. No. 4404021)
For Silencer Select siRNA Positive control:
- Silencer Select GAPDH Positive Control siRNA (Cat. No. 4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
- Silencer Select MALAT1 Positive Control siRNA for non-coding siRNA (Cat. No. 4455877, 5 nmol)
For Silencer Select siRNA negative control:
- Silencer Select Negative Control No. 1 siRNA (Cat. No. 4390843, 5 nmol; 4390844, 40 nmol)
- Silencer Select Negative Control No. 2 siRNA (Cat. No. 4390846, 5 nmol; 4390847, 40 nmol)
If working with Silencer Select siRNA in vivo ready:
- Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250nmol)
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
