Oligo (dT) Primer (50 μM) - FAQs

How does the Anchored Oligo(dT)20 Primer differ from standard oligo(dT) primers?

Anchored Oligo(dT) primers have 2 random bases at the 3' end of the stretch of Ts. The first random base is either an A, G, or C, while the second can be any of the 4 standard nucleotides, i.e., A, T, G or C. The use of anchored oligo(dT) primers results in increased cDNA synthesis yields.