SlideHyb™ Glass Array Hybridization Buffer No. 3 (2 ml tube) - FAQs

查看更多产品信息 SlideHyb™ Glass Array Hybridization Buffer No. 3 (2 ml tube) - FAQs (AM8863)

2 个常见问题解答

Northern印迹可以用来检测小RNA吗？

是的。你可以用15%变性聚丙烯酰胺凝胶对小RNA，例如miRNA和siRNA进行Northern分析。为获得最好的结果，您应该使用针对于短探针的杂交缓冲液，例如ULTRAhyb-Oligo溶液。为获得更灵敏的检测，您可以对RNA样本中小RNA进行富集（例如，使用 mirVana miRNA提取试剂盒或 mirVana PARIS RNA和天然蛋白纯化试剂盒)。当然，使用液相杂交试剂盒例如mirVana miRNA检测试剂盒对RNA进行检测可以提供更大的灵敏度并可以同时检测多个小RNA。

Can Northern blotting be used to detect small RNAs?

Yes. You can use a 15% denaturing polyacrylamide gel for Northern analysis of small RNAs, such as miRNAs and siRNAs. A hyridization buffer optimized for use with short probes, such as ULTRAhyb-Oligo solution, should be used for the best results. For more sensitive detection, enrich the RNA sample for small RNAs (e.g., with the mirVana miRNA Isolation Kit or mirVana PARIS RNA and Native Protein Purification Kit. Of course, using a solution hybridization assay such as the mirVana miRNA Detection Kit to analyze small RNAs can provide much greater sensitivity and allow you to detect multiple small RNAs simultaneously.