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Invitrogen™

甲酰胺(去离子)

经去离子处理,不含核酸酶,以液态形式包装在琥珀色玻璃瓶中的氩气下。
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货号数量
AM93445 x 500 g
AM9342500 g
货号 AM9344
价格(CNY)
5,630.00
飞享价
Ends: 31-Dec-2026
7,128.00
共减 1,498.00 (21%)
Each
数量:
5 x 500 g
价格(CNY)
5,630.00
飞享价
Ends: 31-Dec-2026
7,128.00
共减 1,498.00 (21%)
Each

Ambion formamide has been deionized, is nuclease-free, and comes packaged as a liquid in an amber glass bottle under argon. Supplied in 5 bottles containing 500 g each. Formamide lowers the melting temperature of nucleic acid duplexes and is widely used in applications such as Southern and northern blotting, nuclease protection assays, S1 mapping, and in situ hybridization. For critical applications, it is recommended that the formamide from previously opened bottles be deionized before use.

  • Appearance: Clear, colorless
  • Molecular Weight: 45.04
  • Purity: ≥99.5%
  • Quality Control: The product has been tested for RNase and DNase contamination.
Ambion 甲酰胺经过了去离子处理,不含核酸酶,以液态形式包装在琥珀色玻璃瓶中的氩气下。5瓶装,每瓶 500 g。甲酰胺降低了核酸双链体的解链温度,并广泛用于 Southern 和 Northern 印迹、核酸酶保护测定、S1 图谱分析和原位杂交等应用。对于关键应用,建议在使用前对先前打开的瓶中的甲酰胺进行去离子处理。

外观:透明,无色
分子量:45.04
纯度:>99.5%
质量控制:本产品在 RNase 和 DNase 污染方面经过测试。
仅供科研使用。不可用于诊断程序。
规格
颜色无色
包装类型瓶装
产品线Ambion
产品类型甲酰胺
纯度>99.5%
数量5 x 500 g
形式液体
Unit SizeEach
内容与储存
在 4° 下储存或分装成小份在 -20°C 下储存。

常见问题解答 (FAQ)

What do you recommend for long-term storage of RNA?

For long-term storage, we recommend resuspending the RNA in stabilized formamide and storing at -70 degrees C. To remove the formamide, add 4 volumes ethanol and, if less than 20 µg RNA, also add NaCl to 0.2 M. Precipitate the RNA and use for downstream experiments.

Can a sample buffer with formamide be used with the Invitrogen TBE-Urea system?

There are many sample buffer formulations used, however we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide resulted in fuzzy, indistinct bands.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.