Plant RNA Isolation Aid - FAQs

Q: What kind of Polyvinylpyrrolidone (PVP) do you recommend using with MagMAX Plant DNA and RNA isolation kits since there are different kinds available?

For the MagMAX Plant DNA Isolation Kit, we recommend using PVP M.W. 40,000, Cat. No. J62417.A1 (https://www.thermofisher.com/order/catalog/product/J62417.A1). For the MagMAX Plant RNA Isolation Kit, we recommend using Plant RNA Isolation Aid, Cat. No. AM9690 (https://www.thermofisher.com/order/catalog/product/AM9690).

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2 个常见问题解答

I used the Plant RNA Isolation Aid along with the RNAqueous kit to isolate total RNA from my plant sample. My RNA preparation is too dilute. What do you recommend?

Yields may be improved in some cases by keeping the preparation more concentrated, especially if the tissue has a high water content. Try reducing the amount of lysis solution. Significant errors in weighing small amounts of tissue (<100 mg) can be introduced if the tissue is extremely wet. Tissue samples should be blotted dry just before weighing. Watery tissues may yield more RNA if they are lyophilized before disruption. This increases the ratio of lysis solution to dry-weight tissue.

What kind of Polyvinylpyrrolidone (PVP) do you recommend using with MagMAX Plant DNA and RNA isolation kits since there are different kinds available?

For the MagMAX Plant DNA Isolation Kit, we recommend using PVP M.W. 40,000, Cat. No. J62417.A1 (https://www.thermofisher.com/order/catalog/product/J62417.A1).

For the MagMAX Plant RNA Isolation Kit, we recommend using Plant RNA Isolation Aid, Cat. No. AM9690 (https://www.thermofisher.com/order/catalog/product/AM9690).