Evos Fl Auto, No Obj/no Cube - FAQs

对于EVOS成像系统而言：

•确认灯已打开（很容易检查：在载物台上放一块薄纸）
•确认样品不要太混浊；与校准载玻片或其他薄的单细胞样品载玻片进行对比。
•检查物镜，确认物镜转轮对齐并且物镜完全转动到它的位置。
•对于EVOSFL成像系统：移动光立方的位置
•对于EVOSFL Auto成像系统：检查所有的USB端口，确保显微镜与电脑连接。
• 对于明场设置，检查聚光镜滑块位置，确保聚光镜滑块在预定的位置。

当在Z轴（上和下）聚焦得太高时，物镜会擦到容器支架适配器。这是EVOS FL Auto成像系统启动、仪器启动时移动载物台或切换物镜时遇到的特殊问题。盖玻片矫正物镜的镜筒顶部更加宽和平，尤其是在样品容器边缘成像时，这意味着它们更容易触碰容器支架适配器的边缘，在这些情况下，无法使用物镜在这些区域成像。如果物镜被容器支架适配器‘’卡‘’住了，慢慢的旋转容器支架适配器上的拇指螺丝，将其竖直提起离开载物台，然后再朝着载物台的中心向下移动物镜聚焦。最好的办法是在您的实验室设定一个关机流程，包括将物镜移动到最低的放大倍数，在每天关闭仪器之前向下调整镜头。

物镜镜头在与容器支架适配器的刮擦时会严重损毁。如果发生刮擦，取出物镜，仔细检查物镜——尤其是镜头——是否损坏。 

您可点击此处（https://www.thermofisher.com/us/en/home/products-and-services/services/instrument-qualification-services/instruments-and-services-portal/instrument-software-downloads.html）访问相应的页面。在“细胞成像系统”部分下，找到并点击您的EVOS成像系统的链接。之后您即可发现三个下载链接和若干说明。我们建议您至少每六个月检查一次更新，或者在您的系统出现任何软件方面的小问题时检查一次更新。

只有EVOS FL自动成像系统和EVOS FL 2 自动成像系统具有细胞计数功能。EVOS FL自动成像系统通过内置的仪器固件实现细胞自动计数，而EVOS FL Auto 2成像系统需要单独购买Celleste图像分析软件实现细胞自动计数。< br / > < br / > EVOS XL、EVOS FL和EVOS FL彩色成像系统提供了一个手动细胞计数工具，允许在屏幕图像上标记多达六个不同的标签。

FLoid 细胞成像工作站是一个简单、易用的透射光及三色荧光显微镜。蓝色、绿色和可见红色（Texas Red染料）三种荧光色为固定设置，不可更换为其他滤片组。放大倍数固定设置为20x的物镜；该物镜不可移除或更换。

而EVOS FL成像系统是易于使用的透射光及荧光显微镜，允许用户通过物镜转轮和光立方托盘选择多种放大倍数和荧光检测选项。EVOS FL 成像系统允许使用23种不同的光立方和18种不同的物镜（从2x到100x）。

可以，Countess II FL仪器与EVOS成像系统使用同样的光立方。Countess II仪器不能使用光立方。

您应该根据自己实验室所需的分析复杂程度以及自身是需要透射（彩色）相机成像或荧光（单色）相机成像来选择。在常规/基础分析方面，我们提供用于样品透射光成像（代表性的用于细胞培养仪器）的EVOS XL Core成像系统，或者用于三色荧光成像的FLoid 细胞成像工作站。EVOS XL 成像系统与EVOS XL Core成像系统不同之处在于其配有一个较大的监视器，一个五物镜转轮（EVOS XL Core仪器只有4物镜转轮），能联网，能完成细胞计数。 

如果您需要更多荧光波长的选择，或者细胞计数，延时成像功能，可以考虑EVOS FL 成像系统或者EVOS FL Auto成像系统。EVOS FL Auto成像系统是功能最丰富的产品，具有自动成像和更多特色功能。其拥有彩色和单色相机的选择，甚至可以与我们的EVOS台式培养室系统配合使用。

For the EVOS imaging systems:

Make certain the light is on (easy way to test this: place a piece of thin paper on the stage).
Make certain the sample is not too opaque; compare with a calibration slide or another, thinner or single-cell sample on a slide.
Check the objectives to make certain the turret is in alignment and the objective is completely threaded in its slot.
For the EVOS FL Imaging System: change the position of the light cubes.
For the EVOS FL Auto Imaging System: check the insertion of all USB ports for connectively from scope to computer.
For brightfield settings, check the condenser slider slot; make certain the condenser sliders are completely in place.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Objectives can hit the vessel holder when they are focused too high in the Z axis (up and down). This is a particularly a problem with the EVOS FL Auto Imaging System during instrument start-up, when the stage moves during system initiation, or when changing objectives. Coverslip-corrected objectives tend to be wider and flatter at the top of the barrel, which means that they are more likely to run into the edges of the vessel holder, particularly if you are imaging at the edges of the sample container. In those cases, use of that objective for those areas of the container may not be possible. If the objective if “jammed” by the vessel holder, then carefully unscrew the thumbscrews of the vessel holder and lift it straight off the stage, then move the objective downward in focus and toward the center of the stage. It is a good idea to have a shut-down procedure in your lab that includes moving the objectives to the lowest magnification and focusing downward with course focus prior to turning off the instrument for the day.
An objective can be damaged by scraping against the vessel holder. If this happens, take out the objective and examine it carefully for damage, particularly on the lens.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Here is a page you can go to - https://www.thermofisher.com/us/en/home/products-and-services/services/instrument-qualification-services/instruments-and-services-portal/instrument-software-downloads.html. Look under the “Cell Imaging Systems” section and follow the link for your EVOS imaging system. There you will find the free download link as well as instructions to follow. We recommend you check for updates at least every six months, or if your system seems to have a software glitch of any sort.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Only the EVOS FL Auto Imaging System and EVOS FL Auto 2 Imaging System have an automatic cell counting function. On the EVOS FL Auto Imaging System, automatic cell counting is enabled via built-in instrument firmware whereas on the EVOS FL Auto 2 Imaging System, the Celleste Image Analysis Software needs to be purchased separately to enable automatic cell counting.

The EVOS XL, EVOS FL, and EVOS FL Color imaging systems provide a manual cell counting tool that allows tagging of up to six different labels on the screen image.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The FLoid Cell Imaging Station is a simple, easy-to-use transmitted-light and three-color fluorescence microscope. The three fluorescent colors, blue, green, and visible red (Texas Red dye) are set and cannot be changed to other filter sets. The magnification is also set with a 20x objective; this objective cannot be removed or changed.

On the other hand, the EVOS FL Imaging Systems are easy-to-use transmitted-light and fluorescence microscopes that allow the user access to the objective turret and light cube tray to permit multiple options for magnification and fluorescence detection. The EVOS FL Imaging Systems allow the use of 23 different light cubes and 18 different objectives (from 2x to 100x).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Yes, the Countess II FL instrument uses the same light cubes as the EVOS imaging systems. The Countess II instrument does not use light cubes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

You should choose based upon the complexity of your labs' analyses and whether you need transmitted (color) camera imaging or fluorescent (monochrome) camera imaging, or both. At routine/basic analyses, we offer the EVOS XL Core Imaging System for simple transmitted light imaging (typically used in cell culture facilities) or the FLoid Cell Imaging Station for dedicated three-color fluorescence imaging. The EVOS XL Imaging System differs from the EVOS XL Core Imaging System in that it has a larger monitor, a five objective turret (only four objectives with the EVOS XL Core instrument), can be networked, and can perform cell counting.

If you need more options for fluorescent wavelengths, cell counting, or time-lapse imaging, you may consider the EVOS FL Imaging System or the EVOS FL Auto Imaging System. The EVOS FL Auto Imaging System is our most versatile option, featuring automated imaging and more. It has both color and monochrome camera options, and can even be paired with our EVOS Onstage Incubator system.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.