IL-17 Promotes Differentiation of Splenic LSK(-) Lymphoid Progenitors into B Cells following Plasmodium yoelii Infection.
AuthorsGhosh D, Brown SL, Stumhofer JS
JournalJ Immunol
PubMed ID28733485
Lineage(-)Sca-1(+)c-Kit(-) (LSK(-)) cells are a lymphoid progenitor population that expands in the spleen and preferentially differentiates into mature B cells in response to Plasmodium yoelii infection in mice. Furthermore, LSK(-) derived B cells can subsequently contribute to the ongoing immune response through the generation of parasite-specific Ab-secreting cells, as well ... More
Enhanced Stem Cell Differentiation and Immunopurification of Genome Engineered Human Retinal Ganglion Cells.
AuthorsSluch VM, Chamling X, Liu MM, Berlinicke CA, Cheng J, Mitchell KL, Welsbie DS, Zack DJ
JournalStem Cells Transl Med
PubMed ID29024560
Human pluripotent stem cells have the potential to promote biological studies and accelerate drug discovery efforts by making possible direct experimentation on a variety of human cell types of interest. However, stem cell cultures are generally heterogeneous and efficient differentiation and purification protocols are often lacking. Here, we describe the ... More
OPA1 deficiency promotes secretion of FGF21 from muscle that prevents obesity and insulin resistance.
AuthorsPereira RO, Tadinada SM, Zasadny FM, Oliveira KJ, Pires KMP, Olvera A, Jeffers J, Souvenir R, Mcglauflin R, Seei A, Funari T, Sesaki H, Potthoff MJ, Adams CM, Anderson EJ, Abel ED
JournalEMBO J
PubMed ID28607005
Mitochondrial dynamics is a conserved process by which mitochondria undergo repeated cycles of fusion and fission, leading to exchange of mitochondrial genetic content, ions, metabolites, and proteins. Here, we examine the role of the mitochondrial fusion protein optic atrophy 1 (OPA1) in differentiated skeletal muscle by reducing OPA1 gene expression ... More