MEGAscript™ T7 Transcription Kit, 200 reactions - FAQs

With the MEGAscript T7 Transcription Kit, can the T7 polymerase synthesize RNA from a single-stranded DNA template, containing the T7 promoter sequence, or does it require a double-stranded DNA promoter sequence to work?

The T7 polymerase requires, at the minimum, for the promoter sequence to be double-stranded. The rest of the template does not necessarily have to be double-stranded.

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