Direct Writing of Polymeric Coatings on Magnesium Alloy for Tracheal Stent Applications.
AuthorsPerkins J, Xu Z, Smith C, Roy A, Kumta PN, Waterman J, Conklin D, Desai S,
Journal
PubMed ID25348845
'This paper investigates the direct-write inkjet method for depositing multi-layer coatings of biodegradable polymers on magnesium alloy surface. Immersion studies were conducted on Poly(lactic-co-glycolic) acid (PLGA), polycaprolactone (PCL), and poly-ester urethane urea (PEUU) coatings to determine the corrosion behavior of different samples based on their varying degradation properties. Using the ... More
Preparation and structure of drug-carrying biodegradable microspheres designed for transarterial chemoembolization therapy.
AuthorsWang Y, Benzina A, Molin DG, Akker Nv, Gagliardi M, Koole LH,
Journal
PubMed ID25425276
'Biodegradable poly(D,L-lactic acid) drug-eluting microspheres containing anti-tumor drugs, cisplatin, and sorafenib tosylate have been prepared by the emulsion solvent evaporation method with diameter between 200 and 400 µm. Scanning electron microscopy showed that cisplatin microspheres had smooth surfaces, while sorafenib tosylate microspheres and cisplatin + sorafenib tosylate microspheres were porous ... More
RNAi-mediated knockdown of E2F2 inhibits tumorigenicity of human glioblastoma cells.
AuthorsNakahata AM, Suzuki DE, Rodini CO, Fiuza ML, Okamoto OK,
Journal
PubMed ID25202354
'In a previous genome-wide expression profiling study, we identified E2F2 as a hyperexpressed gene in stem-like cells of distinct glioblastoma multiforme (GBM) specimens. Since the encoded E2F2 transcription factor has been implicated in both tumor suppression and tumor development, we conducted a functional study to investigate the pertinence of E2F2 ... More
A new spontaneously transformed syngeneic model of high-grade serous ovarian cancer with a tumor-initiating cell population.
AuthorsMcCloskey CW, Goldberg RL, Carter LE, Gamwell LF, Al-Hujaily EM, Collins O, Macdonald EA, Garson K, Daneshmand M, Carmona E, Vanderhyden BC,
Journal
PubMed ID24672774
'Improving screening and treatment options for patients with epithelial ovarian cancer has been a major challenge in cancer research. Development of novel diagnostic and therapeutic approaches, particularly for the most common subtype, high-grade serous ovarian cancer (HGSC), has been hampered by controversies over the origin of the disease and a ... More
Ethanol-induced transcriptional activation of programmed cell death 4 (Pdcd4) is mediated by GSK-3ß signaling in rat cortical neuroblasts.
'Ingestion of ethanol (ETOH) during pregnancy induces grave abnormalities in developing fetal brain. We have previously reported that ETOH induces programmed cell death 4 (PDCD4), a critical regulator of cell growth, in cultured fetal cerebral cortical neurons (PCNs) and in the cerebral cortex in vivo and affect protein synthesis as ... More
Sulforaphane reduces vascular inflammation in mice and prevents TNF-a-induced monocyte adhesion to primary endothelial cells through interfering with the NF-?B pathway.
AuthorsNallasamy P, Si H, Babu PV, Pan D, Fu Y, Brooke EA, Shah H, Zhen W, Zhu H, Liu D, Li Y, Jia Z,
Journal
PubMed ID24880493
'Sulforaphane, a naturally occurring isothiocyanate present in cruciferous vegetables, has received wide attention for its potential to improve vascular function in vitro. However, its effect in vivo and the molecular mechanism of sulforaphane at physiological concentrations remain unclear. Here, we report that a sulforaphane concentration as low as 0.5 µM ... More
Phage display discovery of novel molecular targets in glioblastoma-initiating cells.
AuthorsLiu JK, Lubelski D, Schonberg DL, Wu Q, Hale JS, Flavahan WA, Mulkearns-Hubert EE, Man J, Hjelmeland AB, Yu J, Lathia JD, Rich JN,
Journal
PubMed ID24832468
'Glioblastoma is the most common primary intrinsic brain tumor and remains incurable despite maximal therapy. Glioblastomas display cellular hierarchies with self-renewing glioma-initiating cells (GICs) at the apex. To discover new GIC targets, we used in vivo delivery of phage display technology to screen for molecules selectively binding GICs that may ... More
Effect of biodegradability on CXCR4 antagonism, transfection efficacy and antimetastatic activity of polymeric Plerixafor.
AuthorsLi J, Oupický D,
Journal
PubMed ID24726746
'Chemokine receptor CXCR4 and its sole ligand SDF-1 are key players in regulating cancer cell invasion and metastasis. Plerixafor (AMD3100) is a small-molecule CXCR4 antagonist that prevents binding of SDF-1 to CXCR4 and has potential in prevention of cancer metastasis. This study investigates the influence of biodegradability of a recently ... More
Viability characterization of Taxus chinensis plant cell suspension cultures by rapid colorimetric- and image analysis-based techniques.
AuthorsWucherpfennig T, Schulz A, Pimentel JA, Corkidi G, Sieblitz D, Pump M, Gorr G, Schütte K, Wittmann C, Krull R,
Journal
PubMed ID24658794
'For the commercially established process of paclitaxel production with Taxus chinensis plant cell culture, the size of plant cell aggregates and phenotypic changes in coloration during cultivation have long been acknowledged as intangible parameters. So far, the variability of aggregates and coloration of cells are challenging parameters for any viability ... More
Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.
AuthorsEibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM,
Journal
PubMed ID24670060
'With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental ... More
Tauopathy contributes to synaptic and cognitive deficits in a murine model for Alzheimer's disease.
AuthorsStancu IC, Ris L, Vasconcelos B, Marinangeli C, Goeminne L, Laporte V, Haylani LE, Couturier J, Schakman O, Gailly P, Pierrot N, Kienlen-Campard P, Octave JN, Dewachter I,
Journal
PubMed ID24604080
'Tau alterations are now considered an executor of neuronal demise and cognitive dysfunction in Alzheimer''s disease (AD). Mouse models combining amyloidosis and tauopathy and their parental counterparts are important tools to further investigate the interplay of abnormal amyloid-ß (Aß) and Tau species in pathogenesis, synaptic and neuronal dysfunction, and cognitive ... More
Stable luciferase expression does not alter immunologic or in vivo growth properties of GL261 murine glioma cells.
AuthorsClark AJ, Safaee M, Oh T, Ivan ME, Parimi V, Hashizume R, Ozawa T, James CD, Bloch O, Parsa AT,
Journal
PubMed ID25464980
'BackgroundGL261 cells are murine glioma cells that demonstrate proliferation, invasion, and angiogenesis when implanted in syngeneic C57BL/6 mice, providing a highly useful immunocompetent animal model of glioblastoma. Modification of tumor cells for luciferase expression enables non-invasive monitoring of orthotopic tumor growth, and has proven useful for studying glioblastoma response to ... More
The in vivo mechanism of action of CD20 monoclonal antibodies depends on local tumor burden.
AuthorsBoross P, Jansen JH, de Haij S, Beurskens FJ, van der Poel CE, Bevaart L, Nederend M, Golay J, van de Winkel JG, Parren PW, Leusen JH,
JournalHaematologica
PubMed ID21880632
'CD20 monoclonal antibodies are widely used in clinical practice. Antibody-dependent cellular cytotoxicity, complement-dependent cytotoxicity and direct cell death have been suggested to be important effector functions for CD20 antibodies. However, their specific contributions to the in vivo mechanism of action of CD20 immunotherapy have not been well defined. Here we ... More
Unique differentiation profile of mouse embryonic stem cells in rotary and stirred tank bioreactors.
AuthorsFridley KM, Fernandez I, Li MT, Kettlewell RB, Roy K,
JournalTissue Eng Part A
PubMed ID20528675
'Embryonic stem (ES)-cell-derived lineage-specific stem cells, for example, hematopoietic stem cells, could provide a potentially unlimited source for transplantable cells, especially for cell-based therapies. However, reproducible methods must be developed to maximize and scale-up ES cell differentiation to produce clinically relevant numbers of therapeutic cells. Bioreactor-based dynamic culture conditions are ... More
Endocrine disruptors fludioxonil and fenhexamid stimulate miR-21 expression in breast cancer cells.
AuthorsTeng Y, Manavalan TT, Hu C, Medjakovic S, Jungbauer A, Klinge CM,
JournalToxicol Sci
PubMed ID23052036
'Fenhexamid and fludioxonil are antifungal agents used in agricultural applications, which are present at measurable amounts in fruits and vegetables. Fenhexamid and fludioxonil showed endocrine disruptor activity as antiandrogens in an androgen receptor reporter assay in engineered human breast cancer cells. Little is known about how environmental chemicals regulate microRNA ... More
Differential expression of 2',3'-cyclic-nucleotide 3'-phosphodiesterase and neural lineage markers correlate with glioblastoma xenograft infiltration and patient survival.
AuthorsZorniak M, Clark PA, Leeper HE, Tipping MD, Francis DM, Kozak KR, Salamat MS, Kuo JS,
JournalClin Cancer Res
PubMed ID22589395
'Glioblastoma multiforme (GBM) is a poorly treated human brain cancer with few established clinically useful molecular prognostic markers. We characterized glioblastoma stem-like cells (GSC) according to developmental neural lineage markers and correlated their expression with patient survival. Immunoblot array of neural lineage markers classified five independently isolated human GSC lines ... More
Lenalidomide restrains motility and overangiogenic potential of bone marrow endothelial cells in patients with active multiple myeloma.
AuthorsDe Luisi A, Ferrucci A, Coluccia AM, Ria R, Moschetta M, de Luca E, Pieroni L, Maffia M, Urbani A, Di Pietro G, Guarini A, Ranieri G, Ditonno P, Berardi S, Caivano A, Basile A, Cascavilla N, Capalbo S, Quarta G, Dammacco F, Ribatti D, Vacca A,
JournalClin Cancer Res
PubMed ID21307145
'To determine the in vivo and in vitro antiangiogenic power of lenalidomide, a '
Improving catalase-based propelled motor endurance by enzyme encapsulation.
AuthorsSimmchen J, Baeza A, Ruiz-Molina D, Vallet-Regí M,
Journal
PubMed ID24964766
Biocatalytic propulsion is expected to play an important role in the future of micromotors as it might drastically increase the number of available fuelling reactions. However, most of the enzyme-propelled micromotors so far reported still rely on the degradation of peroxide by catalase, in spite of being vulnerable to relatively ... More
Components of the Arabidopsis nuclear pore complex play multiple diverse roles in control of plant growth.
AuthorsParry G,
Journal
PubMed ID25165147
The nuclear pore complex (NPC) is a multisubunit protein conglomerate that facilitates movement of RNA and protein between the nucleus and cytoplasm. Relatively little is known regarding the influence of the Arabidopsis NPC on growth and development. Seedling development, flowering time, nuclear morphology, mRNA accumulation, and gene expression changes in ... More
Inhibitory activity of the isoflavone biochanin A on intracellular bacteria of genus Chlamydia and initial development of a buccal formulation.
AuthorsHanski L, Genina N, Uvell H, Malinovskaja K, Gylfe Å, Laaksonen T, Kolakovic R, Mäkilä E, Salonen J, Hirvonen J, Elofsson M, Sandler N, Vuorela PM,
Journal
PubMed ID25514140
Given the established role of Chlamydia spp. as causative agents of both acute and chronic diseases, search for new antimicrobial agents against these intracellular bacteria is required to promote human health. Isoflavones are naturally occurring phytoestrogens, antioxidants and efflux pump inhibitors, but their therapeutic use is limited by poor water-solubility ... More
Ellagic acid modulates lipid accumulation in primary human adipocytes and human hepatoma Huh7 cells via discrete mechanisms.
AuthorsOkla M, Kang I, Kim da M, Gourineni V, Shay N, Gu L, Chung S,
Journal
PubMed ID25458530
Previously, we have reported that consumption of a muscadine grape phytochemical powder (MGP) decreased lipid accumulation in high-fat fed mice. The aim of this study was to identify the responsible polyphenolic constituents and elucidate the underlying mechanisms. In mice, MGP supplementation significantly reduced visceral fat mass as well as adipocyte ... More
MicroRNA-Mediated Transformation by the Kaposi's Sarcoma-Associated Herpesvirus Kaposin Locus.
AuthorsForte E, Raja AN, Shamulailatpam P, Manzano M, Schipma MJ, Casey JL, Gottwein E,
Journal
PubMed ID25505059
The human oncogenic Kaposi's sarcoma-associated herpesvirus (KSHV) expresses a set of ~20 viral microRNAs (miRNAs). miR-K10a stands out among these miRNAs because its entire stem-loop precursor overlaps the coding sequence for the Kaposin (Kap) A/C proteins. The ectopic expression of KapA has been reported to lead to transformation of rodent ... More
Peptides derived from the copper-binding region of lysyl oxidase exhibit antiangiogeneic properties by inhibiting enzyme activity: an in vitro study.
Despite the rigorous research on abnormal angiogenesis, there is a persistent need for the development of new and efficient therapies against angiogenesis-related diseases. The role of Lysyl oxidase (LOX) in angiogenesis and cancer has been established in prior studies. Copper is known to induce the synthesis of LOX, and hence ... More
Functional characterization of SjB10, an intracellular serpin from Schistosoma japonicum.
SUMMARY Serine protease inhibitors (serpin) play essential roles in many organisms. Mammalian serpins regulate the blood coagulation, fibrinolysis, inflammation and complement activation pathways. In parasitic helminths, serpins are less well characterized, but may also be involved in evasion of the host immune response. In this study, a Schistosoma japonicum serpin ... More
Characterization of a broadly neutralizing monoclonal antibody that targets the fusion domain of group 2 influenza A virus hemagglutinin.
AuthorsTan GS, Lee PS, Hoffman RM, Mazel-Sanchez B, Krammer F, Leon PE, Ward AB, Wilson IA, Palese P,
Journal
PubMed ID25210195
Due to continuous changes to its antigenic regions, influenza viruses can evade immune detection and cause a significant amount of morbidity and mortality around the world. Influenza vaccinations can protect against disease but must be annually reformulated to match the current circulating strains. In the development of a broad-spectrum influenza ... More
Retinoic acid biosynthesis is impaired in human and murine endometriosis.
AuthorsPierzchalski K, Taylor RN, Nezhat C, Jones JW, Napoli JL, Yang G, Kane MA, Sidell N,
Journal
PubMed ID25143356
Endometriosis is characterized by the presence of endometrial glands and stroma in extrauterine sites. Our objective was to determine whether endometriotic lesions (ELs) from women with endometriosis have altered retinoid levels compared with their eutopic endometrium, and to test the hypothesis that defects in all-trans retinoic acid (ATRA) biosynthesis in ... More
Culture bag systems for clinical applications of adult human neural crest-derived stem cells.
Facing the challenging treatment of neurodegenerative diseases as well as complex craniofacial injuries such as those common after cancer therapy, the field of regenerative medicine increasingly relies on stem cell transplantation strategies. Here, neural crest-derived stem cells (NCSCs) offer many promising applications, although scale up of clinical-grade processes prior to ... More
Maternal overnutrition enhances mRNA expression of adipogenic markers and collagen deposition in skeletal muscle of beef cattle fetuses.
AuthorsDuarte MS, Gionbelli MP, Paulino PV, Serão NV, Nascimento CS, Botelho ME, Martins TS, Filho SC, Dodson MV, Guimarães SE, Du M,
Journal
PubMed ID25006073
Twenty-four pregnant Nellore cows were randomly assigned into 2 feeding level groups (control [CTL]; fed 1.0 times the maintenance requirement; n = 12; and overnourished [ON]; fed at 1.5 times the maintenance requirement; n = 12) to evaluate effects of maternal overnutrition on fetal skeletal muscle development. Cows were slaughtered ... More
Trigonelline attenuates the adipocyte differentiation and lipid accumulation in 3T3-L1 cells.
AuthorsIlavenil S, Arasu MV, Lee JC, Kim da H, Roh SG, Park HS, Choi GJ, Mayakrishnan V, Choi KC,
Journal
PubMed ID24369814
Trigonelline is a natural alkaloid mainly found in Trigonella Foenum Graecum (fenugreek) Fabaceae and other edible plants with a variety of medicinal applications. Therefore, we investigated the molecular mechanism of trigonelline (TG) on the inhibition of adipocyte differentiation and lipid accumulation in 3T3-L1 cells. Trigonelline suppressed lipid droplet accumulation in ... More
Utilization of flax fibers for biomedical applications.
AuthorsMichel SA, Vogels RR, Bouvy ND, Knetsch ML, van den Akker NM, Gijbels MJ, van der Marel C, Vermeersch J, Molin DG, Koole LH,
Journal
PubMed ID24039184
Over the past decades, a large number of animal-derived materials have been introduced for several biomedical applications. Surprisingly, the use of plant-based materials has lagged behind. To study the feasibility of plant-derived biomedical materials, we chose flax (Linum usitatissimum). Flax fibers possess excellent physical-mechanical properties, are nonbiodegradable, and there is ... More
ATM suppresses SATB1-induced malignant progression in breast epithelial cells.
AuthorsOrdinario E, Han HJ, Furuta S, Heiser LM, Jakkula LR, Rodier F, Spellman PT, Campisi J, Gray JW, Bissell MJ, Kohwi Y, Kohwi-Shigematsu T,
JournalPLoS One
PubMed ID23251624
SATB1 drives metastasis when expressed in breast tumor cells by radically reprogramming gene expression. Here, we show that SATB1 also has an oncogenic activity to transform certain non-malignant breast epithelial cell lines. We studied the non-malignant MCF10A cell line, which is used widely in the literature. We obtained aliquots from ... More
Inhibition of biofilm formation by esomeprazole in Pseudomonas aeruginosa and Staphylococcus aureus.
AuthorsSingh V, Arora V, Alam MJ, Garey KW,
JournalAntimicrob Agents Chemother
PubMed ID22664967
Staphylococcus aureus and Pseudomonas aeruginosa are common nosocomial pathogens responsible for biofilm-associated infections. Proton pump inhibitors (PPI), such as esomeprazole, may have novel antimicrobial properties. The objective of this study was to assess whether esomeprazole prevents sessile bacterial growth and biofilm formation and whether it may have synergistic killing effects ... More
LuxS mediates iron-dependent biofilm formation, competence, and fratricide in Streptococcus pneumoniae.
AuthorsTrappetti C, Potter AJ, Paton AW, Oggioni MR, Paton JC,
JournalInfect Immun
PubMed ID21875962
During infection, Streptococcus pneumoniae exists mainly in sessile biofilms rather than in planktonic form, except during sepsis. The capacity to form biofilms is believed to be important for nasopharyngeal colonization as well as disease pathogenesis, but relatively little is known about the regulation of this process. Here, we investigated the ... More
Cell-type-specific nuclei purification from whole animals for genome-wide expression and chromatin profiling.
An understanding of developmental processes requires knowledge of transcriptional and epigenetic landscapes at the level of tissues and ultimately individual cells. However, obtaining tissue- or cell-type-specific expression and chromatin profiles for animals has been challenging. Here we describe a method for purifying nuclei from specific cell types of animal models ... More
Essential role of ARID2 protein-containing SWI/SNF complex in tissue-specific gene expression.
AuthorsXu F, Flowers S, Moran E,
JournalJ Biol Chem
PubMed ID22184115
Unfolding of the gene expression program that converts precursor cells to their terminally differentiated counterparts is critically dependent on the nucleosome-remodeling activity of the mammalian SWI/SNF complex. The complex can be powered by either of two alternative ATPases, BRM or BRG1. BRG1 is critical for development and the activation of ... More
Defects in Glanzmann thrombasthenia and LAD-III (LAD-1/v) syndrome: the role of integrin ß1 and ß3 in platelet adhesion to collagen.
Authorsvan de Vijver E, De Cuyper IM, Gerrits AJ, Verhoeven AJ, Seeger K, Gutiérrez L, van den Berg TK, Kuijpers TW,
JournalBlood
PubMed ID22065596
Patients with Glanzmann thrombasthenia or Leukocyte Adhesion Deficiency-III syndrome (LAD-III or LAD-1/variant) present with increased bleeding tendency because of the lack or dysfunction of the fibrinogen receptor GPIIb/IIIa (integrin aIIbß3), respectively. Although the bleeding disorder is more severe in LAD-III patients, classic aggregometry or perfusion of Glanzmann or LAD-III platelets ... More
Inhibitory effect of LXR activation on cell proliferation and cell cycle progression through lipogenic activity.
AuthorsKim KH, Lee GY, Kim JI, Ham M, Won Lee J, Kim JB,
JournalJ Lipid Res
PubMed ID20847297
Liver X receptor (LXR), a sterol-activated nuclear hormone receptor, has been implicated in cholesterol and fatty acid homeostasis via regulation of reverse cholesterol transport and de novo fatty acid synthesis. LXR is also involved in immune responses, including anti-inflammatory action and T cell proliferation. In this study, we demonstrated that ... More
Glucose-based peritoneal dialysis solution suppresses adiponectin synthesis through oxidative stress in an experimental model of peritoneal dialysis.
AuthorsHuh JY, Seo EY, Lee HB, Ha H,
JournalPerit Dial Int
PubMed ID20798331
Accumulation of visceral fat is one of the major risk factors for the development of cardiovascular disease in peritoneal dialysis (PD) patients. Adiponectin, an adipokine commonly regarded as a negative indicator of metabolic disease, is reported to be downregulated in its gene level in end-stage renal disease patients. Since excessive ... More