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EVOS™ Light Cube, Cy™7 - FAQs

查看更多产品信息 EVOS™ Light Cube, Cy™7 - FAQs (AMEP4667)

4 个常见问题解答

GFP光立方和Qdot 525光立方都能够检测绿色荧光，它们的不同之处在哪？

GFP光立方（Ex = 470/22；Em = 510/42）以470 nm为激发中心，前后各延伸11 nm，总的激发范围从459到481 nm。其发射以510 nm为中心，前后各延伸21 nm，总的发射带宽范围从489到531 nm。

Qdot 525光立方（Ex = 445/45; Em = 525/15）以445 nm为激发中心，前后各延伸22.5 nm，总的激发范围从422.5到467.5 nm。其发射以525 nm为中心，前后各延伸7.5 nm，总的发射带宽范围从517.5到532.5 nm。

GFP光立方最适于有效激发从460到480 nm荧光，且可以从蓝绿光到绿光范围内发射。

Qdot 525光立方更适用于在低波长下（高能）激发量子点，且在绿光范围内具有较窄发射。

Which staining dyes can I use with my Countess 3 FL Automated Cell Counter?

EVOS light cubes, which enable fluorescent detection in the Countess 3 FL Automated Cell Counter, are compatible with a broad range of fluorescent dyes. Refer to our selection guide for an extensive list of compatible dyes. We also offer the ready-to-use ReadyCount Stains for easy viability staining.

Find additional tips, troubleshooting help, and resources within our Cell Counting Support Center.

How can I count different types of cells, such as yeast cells and PBMCs, with my Countess 3 Automated Cell Counter?

Countess 3 Automated Cell Counters produce accurate and precise counts across a range of cell types, including human and murine peripheral blood mononuclear cells (PBMCs). Cell types and isolated nuclei smaller than 5 microns in diameter may be counted in FL only mode. Fluorescent staining, compatible EVOS light cubes, and update to the latest software version is required.

For more information on EVOS Light Cubes and compatible dyes, please use the following link:

EVOS Cell Imaging System Accessories

Find additional tips, troubleshooting help, and resources within our Cell Counting Support Center.

What is the difference between the GFP light cube and the Qdot 525 light cube as they both allow detection of green fluorescence?

The GFP light cube (Ex = 470/22; Em = 510/42) has excitation centered at 470 nm covering 11 nm on each side for a total excitation range from 459 to 481 nm. Its emission is centered at 510 nm with 21 nm on each side for a total emission bandpass range from 489 to 531 nm.
The Qdot 525 light cube (Ex = 445/45; Em = 525/15) has excitation at 445 nm with 22.5 nm on each side for a total excitation range from 422.5 to 467.5. Its emission is centered at 525 nm with 7.5 nm on each side for a total emission bandpass range from 517.5 to 532.5.
The GFP light cube is best suited for fluorophores that are efficiently excited from 460 to 480 nm and emission with in the blue-green to green range.
The Qdot 525 light cube is better suited for exciting quantum dots at lower wavelengths (higher energy) and provides a narrower emission in the green range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.