EVOS™ Light Cube, AO Red - FAQs

GFP光立方（Ex = 470/22；Em = 510/42）以470 nm为激发中心，前后各延伸11 nm，总的激发范围从459到481 nm。其发射以510 nm为中心，前后各延伸21 nm，总的发射带宽范围从489到531 nm。

Qdot 525光立方（Ex = 445/45; Em = 525/15）以445 nm为激发中心，前后各延伸22.5 nm，总的激发范围从422.5到467.5 nm。其发射以525 nm为中心，前后各延伸7.5 nm，总的发射带宽范围从517.5到532.5 nm。

GFP光立方最适于有效激发从460到480 nm荧光，且可以从蓝绿光到绿光范围内发射。

Qdot 525光立方更适用于在低波长下（高能）激发量子点，且在绿光范围内具有较窄发射。

The GFP light cube (Ex = 470/22; Em = 510/42) has excitation centered at 470 nm covering 11 nm on each side for a total excitation range from 459 to 481 nm. Its emission is centered at 510 nm with 21 nm on each side for a total emission bandpass range from 489 to 531 nm.
The Qdot 525 light cube (Ex = 445/45; Em = 525/15) has excitation at 445 nm with 22.5 nm on each side for a total excitation range from 422.5 to 467.5. Its emission is centered at 525 nm with 7.5 nm on each side for a total emission bandpass range from 517.5 to 532.5.
The GFP light cube is best suited for fluorophores that are efficiently excited from 460 to 480 nm and emission with in the blue-green to green range.
The Qdot 525 light cube is better suited for exciting quantum dots at lower wavelengths (higher energy) and provides a narrower emission in the green range.

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