EVOS™ 20X 物镜,萤石,盖玻片校正
EVOS™ 20X 物镜,萤石,盖玻片校正

EVOS™ 20X 物镜,萤石,盖玻片校正

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a coverslip-corrected objective that is optimized for了解更多信息
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货号 AMEP4698
价格(CNY)
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This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 20X
• Numerical Aperture: 0.50
• Working Distance: 2.5 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories
For Research Use Only. Not for use in diagnostic procedures.
规格
焦距2.5 mm
适用于(设备)EVOS™ XL Core 成像系统、EVOS™ FL 彩色成像系统、EVOS™ XL 成像系统、EVOS™ FL 成像系统、EVOS™ FL 自动成像系统
透镜类型平场萤石
放大20X
数值孔径0.50
数量各 1 个
产品线EVOS:
类型物镜
Unit SizeEach

常见问题解答 (FAQ)

我在使用EVOS成像系统时,物镜会擦到载物台容器支架适配器的边缘,如何纠正这一情况?

当在Z轴(上和下)聚焦得太高时,物镜会擦到容器支架适配器。这是EVOS FL Auto成像系统启动、仪器启动时移动载物台或切换物镜时遇到的特殊问题。盖玻片矫正物镜的镜筒顶部更加宽和平,尤其是在样品容器边缘成像时,这意味着它们更容易触碰容器支架适配器的边缘,在这些情况下,无法使用物镜在这些区域成像。如果物镜被容器支架适配器‘’卡‘’住了,慢慢的旋转容器支架适配器上的拇指螺丝,将其竖直提起离开载物台,然后再朝着载物台的中心向下移动物镜聚焦。最好的办法是在您的实验室设定一个关机流程,包括将物镜移动到最低的放大倍数,在每天关闭仪器之前向下调整镜头。

物镜镜头在与容器支架适配器的刮擦时会严重损毁。如果发生刮擦,取出物镜,仔细检查物镜——尤其是镜头——是否损坏。 

当我尝试采用EVOS FL Auto成像系统进行聚焦时,物镜一直不停地在样品上移动,对此该如何处理?

如果镜头一直在样品上移动,可能是对焦太快且丢失焦平面(如果是手动聚焦)的问题,或者是物镜校准的问题(如果使用自动聚焦)。最好使用系统自带的FL Auto校准载玻片校准物镜。首先检查看您的物镜是长工作距离(LWD)物镜或盖玻片矫正(CC)物镜。如果是盖玻片矫正(CC)物镜,则只能采用超短的工作距离,通过薄盖玻片进行成像,不能通过载玻片、微孔板或培养皿内的塑料底进行成像。如果使用高放大倍数或者油镜,利用肉眼观察,向上移动物镜接触到样品的底部,然后缓慢地朝远离样品的方向移动物镜,进一步聚焦。 物镜镜头在与样品的刮擦中会受到严重的损毁。如果发生刮擦,检查物镜的损坏情况。

适用于EVOS细胞成像系统的物镜有各种不同的规格:Plan Achromat,Plan Fluorite和Plan Apochromat。在选择物镜时,我需要考虑哪些因素?

•当颜色和焦距已被标准校准时,对于一般的使用,Plan Achromat物镜是绝佳的;这些适用于放大倍数要求低(2x到4x)的样品。推荐Plan Achromat物镜用于基本视野明亮的显微镜和简单荧光检测。
•Plan Fluorite物镜为明亮的荧光信号和高对比度提供了更高水平的分辨率。推荐基本的荧光成像以及更大放大倍数的明场显微镜使用这些物镜。
•Plan Apochromat物镜提供了最高水平的分辨率、荧光亮度、对比度和色度校准。如果您需要成像非常小的结构且需要高对比度和亮度,Plan Apochromat物镜是最好的选择。

EVOS 成像系统的“长工作距离”(LWD)物镜和“盖玻片矫正”(CC)物镜的差别在哪里?

所有的EVOS成像系统都是倒置显微镜。对CC物镜来说,盖玻片必须朝下,朝向物镜,因为镜头有一个仅适用于薄玻璃盖玻片或塑料盖玻片的短工作距离。LWD物镜设计用于观察微孔板、皮氏培养皿或者细胞培养瓶底部;这些物镜镜头的长工作距离适应于较厚的材料,诸如多种容器的塑料底。 

I'm using an EVOS imaging system and my objective is rubbing up against the edge of the vessel holder of my stage. How can I correct this?

Objectives can hit the vessel holder when they are focused too high in the Z axis (up and down). This is a particularly a problem with the EVOS FL Auto Imaging System during instrument start-up, when the stage moves during system initiation, or when changing objectives. Coverslip-corrected objectives tend to be wider and flatter at the top of the barrel, which means that they are more likely to run into the edges of the vessel holder, particularly if you are imaging at the edges of the sample container. In those cases, use of that objective for those areas of the container may not be possible. If the objective if “jammed” by the vessel holder, then carefully unscrew the thumbscrews of the vessel holder and lift it straight off the stage, then move the objective downward in focus and toward the center of the stage. It is a good idea to have a shut-down procedure in your lab that includes moving the objectives to the lowest magnification and focusing downward with course focus prior to turning off the instrument for the day.
An objective can be damaged by scraping against the vessel holder. If this happens, take out the objective and examine it carefully for damage, particularly on the lens.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.