The existence of a leukocyte complement system is suggested by the findings that certain lymphocytes and mononuclear phagocytes activate complement, express membrane receptors specific for complement activation fragments, and synthesize complement proteins. In this review, attention is directed toward studies indicating that complement proteins may be expressed on the surface ... More
DEPArray™ system: An automatic image-based sorter for isolation of pure circulating tumor cells.
AuthorsDi Trapani M, Manaresi N, Medoro G,
JournalCytometry A
PubMed ID30551261
'Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single-cell level can inform on the genomic landscape of a tumor. ... More
2D or 3D? How cell motility measurements are conserved across dimensions in vitro and translate in vivo.
AuthorsGalarza S, Kim H, Atay N, Peyton SR, Munson JM,
JournalBioeng Transl Med
PubMed ID31989037
'Cell motility is a critical aspect of several processes, such as wound healing and immunity; however, it is dysregulated in cancer. Current limitations of imaging tools make it difficult to study cell migration in vivo. To overcome this, and to identify drivers from the microenvironment that regulate cell migration, bioengineers ... More
Prostate Stroma Increases the Viability and Maintains the Branching Phenotype of Human Prostate Organoids.
AuthorsRichards Z, McCray T, Marsili J, Zenner ML, Manlucu JT, Garcia J, Kajdacsy-Balla A, Murray M, Voisine C, Murphy AB, Abdulkadir SA, Prins GS, Nonn L,
JournaliScience
PubMed ID30735898
'The fibromuscular stroma of the prostate regulates normal epithelial differentiation and contributes to carcinogenesis in vivo. We developed and characterized a human 3D prostate organoid co-culture model that incorporates prostate stroma. Primary prostate stromal cells increased organoid formation and directed organoid morphology into a branched acini structure similar to what is ... More
Amyloid ß-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death.
AuthorsChoi H, Kim C, Song H, Cha MY, Cho HJ, Son SM, Kim HJ, Mook-Jung I,
JournalAging Cell
PubMed ID30515991
'Alzheimer''s disease (AD) is an age-related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death. O-linked ß-N-acetyl glucosamine (O-GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD. However, the link between altered O-GlcNAc modification and neuronal cell death ... More
Properties of viable lyopreserved amnion are equivalent to viable cryopreserved amnion with the convenience of ambient storage.
AuthorsDhall S, Sathyamoorthy M, Kuang JQ, Hoffman T, Moorman M, Lerch A, Jacob V, Sinclair SM, Danilkovitch A,
JournalPLoS One
PubMed ID30278042
Human amniotic membrane (AM) has a long history of clinical use for wound treatment. AM serves as a wound protective barrier maintaining proper moisture. AM is anti-inflammatory, anti-microbial and antifibrotic, and supports angiogenesis, granulation tissue formation and wound re-epithelialization. These properties of AM are attributed to its native extracellular matrix, ... More
Optimizing the Conditions and Use of Synthetic Matrix for Three-Dimensional In Vitro Retinal Differentiation from Mouse Pluripotent Cells.
AuthorsPerepelkina T, Kegeles E, Baranov P,
JournalTissue Eng Part C Methods
PubMed ID31195897
The development of retinal regenerative therapies relies on the reproducible and renewable source of retinal neurons for drug discovery and cell transplantation. Three-dimensional approach for retinal differentiation from pluripotent cells recently emerged as the robust strategy for retinal tissue differentiation. In this work, we present the combination of optimized conditions ... More
Inhibition of dendritic spine extension through acrolein conjugation with a-, ß-tubulin proteins.
AuthorsUemura T, Suzuki T, Ko K, Watanabe K, Dohmae N, Sakamoto A, Terui Y, Toida T, Kashiwagi K, Igarashi K,
JournalInt J Biochem Cell Biol
PubMed ID31150838
We have recently found that conjugation of acrolein with a 50?kDa protein(s) is strongly associated with tissue damage during brain infarction. In the current study, the identity and function of the 50?kDa protein(s) conjugated with acrolein during brain infarction were investigated. The 50?kDa protein(s) conjugated with acrolein were identified as ... More
A Viable Lyopreserved Amniotic Membrane Modulates Diabetic Wound Microenvironment and Accelerates Wound Closure.
AuthorsDhall S, Hoffman T, Sathyamoorthy M, Lerch A, Jacob V, Moorman M, Kuang JQ, Danilkovitch A,
JournalAdv Wound Care (New Rochelle)
PubMed ID31346490
Objective: Wound healing is a complex process involving the dynamic interplay of various types of cells and bioactive factors. Impaired wound healing is characterized by a loss in synchronization of the process, resulting in non-healing chronic wounds. Human amniotic membrane (AM) has been shown to be effective in the management ... More
Polymicrobial synergy within oral biofilm promotes invasion of dendritic cells and survival of consortia members.
AuthorsEl-Awady A, de Sousa Rabelo M, Meghil MM, Rajendran M, Elashiry M, Stadler AF, Foz AM, Susin C, Romito GA, Arce RM, Cutler CW,
JournalNPJ Biofilms Microbiomes
PubMed ID30911403
Years of human microbiome research have confirmed that microbes rarely live or function alone, favoring diverse communities. Yet most experimental host-pathogen studies employ single species models of infection. Here, the influence of three-species oral microbial consortium on growth, virulence, invasion and persistence in dendritic cells (DCs) was examined experimentally in ... More
Different signaling and functionality of Rac1 and Rac1b in the progression of lung adenocarcinoma.
AuthorsSeiz JR, Klinke J, Scharlibbe L, Lohfink D, Heipel M, Ungefroren H, Giehl K, Menke A,
JournalBiol Chem
PubMed ID31811797
Rac1 is a ubiquitously expressed Rho GTPase and an important regulator of the actin cytoskeleton. Its splice variant Rac1b exhibits a 19-amino acid (aa) in-frame insertion and is predominantly active. Both proteins were described in tumorigenesis or metastasis. We investigated the contribution of Rac1 and Rac1b to tumor progression of ... More