'Huntington disease (HD) is an inherited, fatal neurodegenerative disorder caused by a CAG repeat expansion in the huntingtin gene. The mutant protein causes neuronal dysfunction and degeneration resulting in motor dysfunction, cognitive decline, and psychiatric disturbances. Currently, there is no disease altering treatment, and symptomatic therapy has limited benefit. The ... More
Synthesis and characterization of antifouling poly(N-acryloylaminoethoxyethanol) with ultralow protein adsorption and cell attachment.
AuthorsChen H, Zhang M, Yang J, Zhao C, Hu R, Chen Q, Chang Y, Zheng J,
Journal
PubMed ID25127733
'Rational design of effective antifouling polymers is challenging but important for many fundamental and applied applications. Herein we synthesize and characterize an N-acryloylaminoethoxyethanol (AAEE) monomer, which integrates three hydrophilic groups of hydroxyl, amide, and ethylene glycol in the same material. AAEE monomers were further grafted and polymerized on gold substrates ... More
Use of in vitro human keratinocyte models to study the effect of cooling on chemotherapy drug-induced cytotoxicity.
AuthorsAl-Tameemi W, Dunnill C, Hussain O, Komen MM, van den Hurk CJ, Collett A, Georgopoulos NT,
Journal
PubMed ID25091624
'A highly distressing side-effect of cancer chemotherapy is chemotherapy-induced alopecia (CIA). Scalp cooling remains the only treatment for CIA, yet there is no experimental evidence to support the cytoprotective capacity of cooling. We have established a series of in vitro models for the culture of human keratinocytes under conditions where ... More
A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells.
AuthorsXiang P, Wu KC, Zhu Y, Xiang L, Li C, Chen DL, Chen F, Xu G, Wang A, Li M, Jin ZB,
Journal
PubMed ID25220295
'Various artificial membranes have been used as scaffolds for retinal pigment epithelium cells (RPE) for monolayer reconstruction, however, long-term cell viability and functionality are still largely unknown. This study aimed to construct an ultrathin porous nanofibrous film to mimic Bruch''s membrane, and in particular to investigate human RPE cell responses ... More
Recurrent PTPRB and PLCG1 mutations in angiosarcoma.
AuthorsBehjati S, Tarpey PS, Sheldon H, Martincorena I, Van Loo P, Gundem G, Wedge DC, Ramakrishna M, Cooke SL, Pillay N, Vollan HK, Papaemmanuil E, Koss H, Bunney TD, Hardy C, Joseph OR, Martin S, Mudie L, Butler A, Teague JW, Patil M, Steers G, Cao Y, Gumbs C, Ingram D, Lazar AJ, Little L, Mahadeshwar H, Protopopov A, Al Sannaa GA, Seth S, Song X, Tang J, Zhang J, Ravi V, Torres KE, Khatri B, Halai D, Roxanis I, Baumhoer D, Tirabosco R, Amary MF, Boshoff C, McDermott U, Katan M, Stratton MR, Futreal
Journal
PubMed ID24633157
'Angiosarcoma is an aggressive malignancy that arises spontaneously or secondarily to ionizing radiation or chronic lymphoedema. Previous work has identified aberrant angiogenesis, including occasional somatic mutations in angiogenesis signaling genes, as a key driver of angiosarcoma. Here we employed whole-genome, whole-exome and targeted sequencing to study the somatic changes underpinning ... More
Improved repair of dermal wounds in mice lacking microRNA-155.
Authorsvan Solingen C, Araldi E, Chamorro-Jorganes A, Fernández-Hernando C, Suárez Y,
Journal
PubMed ID24636235
'Wound healing is a well-regulated but complex process that involves haemostasis, inflammation, proliferation and maturation. Recent reports suggest that microRNAs (miRs) play important roles in dermal wound healing. In fact, miR deregulation has been linked with impaired wound repair. miR-155 has been shown to be induced by inflammatory mediators and ... More
Extraluminal helicoidal stretch (Helixtretch): a novel method of intestinal lengthening.
AuthorsDionigi B, Brazzo J, Connors JP, Ahmed A, Fisher JG, Zurakowski D, Fauza DO,
Journal
PubMed ID25487484
'We sought to test a novel, extraluminal method of intestinal lengthening that precludes violation of the intestinal wall. Sprague-Dawley rats (n=45) with size-matched bowel segments isolated by Roux-en-Y reconstruction were divided into three groups. Group 1 (n=14) had no further manipulations. In Groups 2 (n=12) and 3 (n=19), the isolated ... More
Prolactin-induced prostate tumorigenesis links sustained Stat5 signaling with the amplification of basal/stem cells and emergence of putative luminal progenitors.
AuthorsSackmann-Sala L, Chiche A, Mosquera-Garrote N, Boutillon F, Cordier C, Pourmir I, Pascual-Mathey L, Kessal K, Pigat N, Camparo P, Goffin V,
Journal
PubMed ID25193592
'Current androgen ablation therapies for prostate cancer are initially successful, but the frequent development of castration resistance urges the generation of alternative therapies and represents an important health concern. Prolactin/signal transducer and activator of transcription 5 (STAT5) signaling is emerging as a putative target for alternative treatment for prostate cancer. ... More
Epstein-Barr virus latent membrane protein 2A enhances MYC-driven cell cycle progression in a mouse model of B lymphoma.
AuthorsFish K, Chen J, Longnecker R,
Journal
PubMed ID24174629
'Elevated expression of MYC is a shared property of many human cancers. Epstein-Barr virus (EBV) has been associated with lymphoid malignancies, yet collaborative roles between MYC and EBV in lymphomagenesis are unclear. EBV latent membrane protein 2A (LMP2A) functions as a B-cell receptor (BCR) mimic known to provide survival signals ... More
The in vivo mechanism of action of CD20 monoclonal antibodies depends on local tumor burden.
AuthorsBoross P, Jansen JH, de Haij S, Beurskens FJ, van der Poel CE, Bevaart L, Nederend M, Golay J, van de Winkel JG, Parren PW, Leusen JH,
JournalHaematologica
PubMed ID21880632
'CD20 monoclonal antibodies are widely used in clinical practice. Antibody-dependent cellular cytotoxicity, complement-dependent cytotoxicity and direct cell death have been suggested to be important effector functions for CD20 antibodies. However, their specific contributions to the in vivo mechanism of action of CD20 immunotherapy have not been well defined. Here we ... More
Unique differentiation profile of mouse embryonic stem cells in rotary and stirred tank bioreactors.
AuthorsFridley KM, Fernandez I, Li MT, Kettlewell RB, Roy K,
JournalTissue Eng Part A
PubMed ID20528675
'Embryonic stem (ES)-cell-derived lineage-specific stem cells, for example, hematopoietic stem cells, could provide a potentially unlimited source for transplantable cells, especially for cell-based therapies. However, reproducible methods must be developed to maximize and scale-up ES cell differentiation to produce clinically relevant numbers of therapeutic cells. Bioreactor-based dynamic culture conditions are ... More
Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
AuthorsMiles B, Zakhary I, El-Awady A, Scisci E, Carrion J, O'Neill JC, Rawlings A, Stern JK, Susin C, Cutler CW,
Journal
PubMed ID24126519
'Several intracellular pathogens, including a key etiological agent of chronic periodontitis, Porphyromonas gingivalis, infect blood myeloid dendritic cells (mDCs). This infection results in pathogen dissemination to distant inflammatory sites (i.e., pathogen trafficking). The alteration in chemokine-chemokine receptor expression that contributes to this pathogen trafficking function, particularly toward sites of neovascularization ... More
Endocrine disruptors fludioxonil and fenhexamid stimulate miR-21 expression in breast cancer cells.
AuthorsTeng Y, Manavalan TT, Hu C, Medjakovic S, Jungbauer A, Klinge CM,
JournalToxicol Sci
PubMed ID23052036
'Fenhexamid and fludioxonil are antifungal agents used in agricultural applications, which are present at measurable amounts in fruits and vegetables. Fenhexamid and fludioxonil showed endocrine disruptor activity as antiandrogens in an androgen receptor reporter assay in engineered human breast cancer cells. Little is known about how environmental chemicals regulate microRNA ... More
Differential expression of 2',3'-cyclic-nucleotide 3'-phosphodiesterase and neural lineage markers correlate with glioblastoma xenograft infiltration and patient survival.
AuthorsZorniak M, Clark PA, Leeper HE, Tipping MD, Francis DM, Kozak KR, Salamat MS, Kuo JS,
JournalClin Cancer Res
PubMed ID22589395
'Glioblastoma multiforme (GBM) is a poorly treated human brain cancer with few established clinically useful molecular prognostic markers. We characterized glioblastoma stem-like cells (GSC) according to developmental neural lineage markers and correlated their expression with patient survival. Immunoblot array of neural lineage markers classified five independently isolated human GSC lines ... More
Lenalidomide restrains motility and overangiogenic potential of bone marrow endothelial cells in patients with active multiple myeloma.
AuthorsDe Luisi A, Ferrucci A, Coluccia AM, Ria R, Moschetta M, de Luca E, Pieroni L, Maffia M, Urbani A, Di Pietro G, Guarini A, Ranieri G, Ditonno P, Berardi S, Caivano A, Basile A, Cascavilla N, Capalbo S, Quarta G, Dammacco F, Ribatti D, Vacca A,
JournalClin Cancer Res
PubMed ID21307145
'To determine the in vivo and in vitro antiangiogenic power of lenalidomide, a '
DEPTOR regulates vascular endothelial cell activation and proinflammatory and angiogenic responses.
'The maintenance of normal tissue homeostasis and the prevention of chronic inflammatory disease are dependent on the active process of inflammation resolution. In endothelial cells (ECs), proinflammation results from the activation of intracellular signaling responses and/or the inhibition of endogenous regulatory/pro-resolution signaling networks that, to date, are poorly defined. In ... More
Etoposide selectively ablates activated T cells to control the immunoregulatory disorder hemophagocytic lymphohistiocytosis.
AuthorsJohnson TS, Terrell CE, Millen SH, Katz JD, Hildeman DA, Jordan MB,
Journal
PubMed ID24259502
Hemophagocytic lymphohistiocytosis (HLH) is an inborn disorder of immune regulation caused by mutations affecting perforin-dependent cytotoxicity. Defects in this pathway impair negative feedback between cytotoxic lymphocytes and APCs, leading to prolonged and pathologic activation of T cells. Etoposide, a widely used chemotherapeutic drug that inhibits topoisomerase II, is the mainstay ... More
Silencing the Menkes Copper-Transporting ATPase (Atp7a) Gene in Rat Intestinal Epithelial (IEC-6) Cells Increases Iron Flux via Transcriptional Induction of Ferroportin 1 (Fpn1).
AuthorsGulec S, Collins JF,
Journal
PubMed ID24174620
The Menkes copper-transporting ATPase (Atp7a) gene is induced in rat duodenum during iron deficiency, consistent with copper accumulation in the intestinal mucosa and liver. To test the hypothesis that ATP7A influences intestinal iron metabolism, the Atp7a gene was silenced in rat intestinal epithelial (IEC-6) cells using short hairpin RNA (shRNA) ... More
Inhibiting heat shock protein 90 (HSP90) limits the formation of liver cysts induced by conditional deletion of Pkd1 in mice.
AuthorsSmithline ZB, Nikonova AS, Hensley HH, Cai KQ, Egleston BL, Proia DA, Seeger-Nukpezah T, Golemis EA,
Journal
PubMed ID25474361
Polycystic liver disease (PLD) occurs in 75-90% of patients affected by autosomal dominant polycystic kidney disease (ADPKD), which affects 1:400-1,000 adults and arises from inherited mutations in the PKD1 or PKD2 genes. PLD can lead to bile duct obstructions, infected or bleeding cysts, and hepatomegaly, which can diminish quality of ... More
Nitric oxide mediates metabolic coupling of omentum-derived adipose stroma to ovarian and endometrial cancer cells.
AuthorsSalimian Rizi B, Caneba C, Nowicka A, Nabiyar AW, Liu X, Chen K, Klopp A, Nagrath D,
Journal
PubMed ID25425006
Omental adipose stromal cells (O-ASC) are a multipotent population of mesenchymal stem cells contained in the omentum tissue that promote endometrial and ovarian tumor proliferation, migration, and drug resistance. The mechanistic underpinnings of O-ASCs' role in tumor progression and growth are unclear. Here, we propose a novel nitric oxide (NO)-mediated ... More
The role of TLR8 signaling in acute myeloid leukemia differentiation.
AuthorsIgnatz-Hoover JJ, Wang H, Moreton SA, Chakrabarti A, Agarwal MK, Sun K, Gupta K, Wald DN,
Journal
PubMed ID25283842
Acute myeloid leukemia (AML) is an aggressive disease with a poor 5-year survival of 21% that is characterized by the differentiation arrest of immature myeloid cells. For a rare subtype of AML (acute promyeloctyic leukemia, 5-10% of cases), all-trans retinoic acid therapy removes the differentiation block, yielding over a 90% ... More
Antifouling gold surfaces grafted with aspartic acid and glutamic acid based zwitterionic polymer brushes.
AuthorsLi W, Liu Q, Liu L,
Journal
PubMed ID25262768
We report two new amino acid based antifouling zwitterionic polymers, poly(N(4)-(2-methacrylamidoethyl)asparagine) (pAspAA) and poly(N(5)-(2-methacrylamidoethyl)glutamine) (pGluAA). The vinyl monomers were developed from aspartic acid and glutamic acid. Surface-initiated photoiniferter-mediated polymerization was employed to graft polymer brushes from gold surfaces. Different thickness of polymer brushes was controlled by varying UV irradiation time. ... More
An AAVS1-targeted minigene platform for correction of iPSCs from all five types of chronic granulomatous disease.
AuthorsMerling RK, Sweeney CL, Chu J, Bodansky A, Choi U, Priel DL, Kuhns DB, Wang H, Vasilevsky S, De Ravin SS, Winkler T, Dunbar CE, Zou J, Zarember KA, Gallin JI, Holland SM, Malech HL,
Journal
PubMed ID25288370
There are five genetic forms of chronic granulomatous disease (CGD), resulting from mutations in any of five subunits of phagocyte oxidase, an enzyme complex in neutrophils, monocytes, and macrophages that produces microbicidal reactive oxygen species. We generated induced pluripotent stem cells (iPSCs) from peripheral blood CD34(+) hematopoietic stem cells of ... More
The oncogenic MicroRNA Hsa-miR-155-5p targets the transcription factor ELK3 and links it to the hypoxia response.
AuthorsRobertson ED, Wasylyk C, Ye T, Jung AC, Wasylyk B,
Journal
PubMed ID25401928
The molecular response to hypoxia is a critical cellular process implicated in cancer, and a target for drug development. The activity of the major player, HIF1a, is regulated at different levels by various factors, including the transcription factor ELK3. The molecular mechanisms of this intimate connection remain largely unknown. Whilst ... More
Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis.
AuthorsSakthivel KM, Guruvayoorappan C,
Journal
PubMed ID25243883
Traditionally, Biophytum sensitivum (L.) DC (Oxalidaceae) is used in Indian medicine to treat diseases include stomachache, convulsions, cramps, inflammation, and ulcer. The present study examines the effect of aerial parts of B. sensitivum (methanol extract) on a murine model of ulcerative colitis (UC). UC was induced by intracolonic injection of ... More
Role of Bruton's tyrosine kinase (BTK) in growth and metastasis of INA6 myeloma cells.
AuthorsBam R, Venkateshaiah SU, Khan S, Ling W, Randal SS, Li X, Zhang Q, van Rhee F, Barlogie B, Epstein J, Yaccoby S,
Journal
PubMed ID25083818
Bruton's tyrosine kinase (BTK) and the chemokine receptor CXCR4 are linked in various hematologic malignancies. The aim of the study was to understand the role of BTK in myeloma cell growth and metastasis using the stably BTK knockdown luciferase-expressing INA6 myeloma line. BTK knockdown had reduced adhesion to stroma and ... More
Protective effect of Acacia ferruginea against ulcerative colitis via modulating inflammatory mediators, cytokine profile and NF-?B signal transduction pathways.
AuthorsSakthivel KM, Guruvayoorappan C,
Journal
PubMed ID24941292
In the present study, we evaluated the protective effect of A. ferruginea extract against ulcerative colitis (UC). Male Wistar rats received A. ferruginea extract (10 mg/kg body weight) or sulfasalazine (100 mg/kg body weight) for 5 consecutive days before inducing UC via intrarectal acetic acid (3%) administration. Colonic mucosal injury ... More
Two amino acid-based superlow fouling polymers: poly(lysine methacrylamide) and poly(ornithine methacrylamide).
AuthorsLiu Q, Li W, Singh A, Cheng G, Liu L,
Journal
PubMed ID24613545
We developed and investigated two new antifouling zwitterionic polymers, poly(lysine methacrylamide) (pLysAA) and poly(ornithine methacrylamide) (pOrnAA), both derived from natural amino acids - lysine and ornithine, respectively. The pLysAA and pOrnAA brushes were grafted on gold via the surface-initiated photoiniferter-mediated polymerization, with the polymer film thickness controlled by the UV-irradiation ... More
High ionic strength narrows the population of sites participating in protein ion-exchange adsorption: a single-molecule study.
AuthorsKisley L, Chen J, Mansur AP, Dominguez-Medina S, Kulla E, Kang MK, Shuang B, Kourentzi K, Poongavanam MV, Dhamane S, Willson RC, Landes CF,
Journal
PubMed ID24751557
The retention and elution of proteins in ion-exchange chromatography is routinely controlled by adjusting the mobile phase salt concentration. It has repeatedly been observed, as judged from adsorption isotherms, that the apparent heterogeneity of adsorption is lower at more-eluting, higher ionic strength. Here, we present an investigation into the mechanism ... More
Probing the structural dependence of carbon space lengths of poly(N-hydroxyalkyl acrylamide)-based brushes on antifouling performance.
AuthorsYang J, Zhang M, Chen H, Chang Y, Chen Z, Zheng J,
Journal
PubMed ID24964712
Numerous biocompatible antifouling polymers have been developed for a wide variety of fundamental and practical applications in drug delivery, biosensors, marine coatings, and many other areas. Several antifouling mechanisms have been proposed, but the exact relationship among molecular structure, surface hydration property, and antifouling performance of antifouling polymers still remains ... More
Patient-derived skeletal dysplasia induced pluripotent stem cells display abnormal chondrogenic marker expression and regulation by BMP2 and TGFß1.
Skeletal dysplasias (SDs) are caused by abnormal chondrogenesis during cartilage growth plate differentiation. To study early stages of aberrant cartilage formation in vitro, we generated the first induced pluripotent stem cells (iPSCs) from fibroblasts of an SD patient with a lethal form of metatropic dysplasia, caused by a dominant mutation ... More
Characterization of African bat henipavirus GH-M74a glycoproteins.
AuthorsWeis M, Behner L, Hoffmann M, Krüger N, Herrler G, Drosten C, Drexler JF, Dietzel E, Maisner A,
Journal
PubMed ID24296468
In recent years, novel henipavirus-related sequences have been identified in bats in Africa. To evaluate the potential of African bat henipaviruses to spread in non-bat mammalian cells, we compared the biological functions of the surface glycoproteins G and F of the prototype African henipavirus GH-M74a with those of the glycoproteins ... More
Somatic cells with a heavy mitochondrial DNA mutational load render induced pluripotent stem cells with distinct differentiation defects.
AuthorsWahlestedt M, Ameur A, Moraghebi R, Norddahl GL, Sten G, Woods NB, Bryder D,
Journal
PubMed ID24446123
It has become increasingly clear that several age-associated pathologies associate with mutations in the mitochondrial genome. Experimental modeling of such events has revealed that acquisition of mitochondrial DNA (mtDNA) damage can impair respiratory function and, as a consequence, can lead to widespread decline in cellular function. This includes premature aging ... More
Contrasting effect of perlecan on adipogenic and osteogenic differentiation of mesenchymal stem cells in vitro.
AuthorsNakamura R, Nakamura F, Fukunaga S,
Journal
PubMed ID24000897
Perlecan, a basement membrane component, shows diverse functions in different organs and tissues. However, the role of perlecan in differentiation of mesenchymal stem cells (MSCs) has been barely investigated. In this study, we examined the effect of perlecan on adipogenic and osteogenic differentiation of MSCs in vitro by adding extrinsic ... More
Identification of a stem-like cell population by exposing metastatic breast cancer cell lines to repetitive cycles of hypoxia and reoxygenation.
AuthorsLouie E, Nik S, Chen JS, Schmidt M, Song B, Pacson C, Chen XF, Park S, Ju J, Chen EI,
JournalBreast Cancer Res
PubMed ID21067584
The irregular vasculature of solid tumors creates hypoxic regions, which are characterized by cyclic periods of hypoxia and reoxygenation. Accumulated evidence suggests that chronic and repetitive exposure to hypoxia and reoxygenation seem to provide an advantage to tumor growth. Although the development of hypoxia tolerance in tumors predicts poor prognosis, ... More
Inhibition of biofilm formation by esomeprazole in Pseudomonas aeruginosa and Staphylococcus aureus.
AuthorsSingh V, Arora V, Alam MJ, Garey KW,
JournalAntimicrob Agents Chemother
PubMed ID22664967
Staphylococcus aureus and Pseudomonas aeruginosa are common nosocomial pathogens responsible for biofilm-associated infections. Proton pump inhibitors (PPI), such as esomeprazole, may have novel antimicrobial properties. The objective of this study was to assess whether esomeprazole prevents sessile bacterial growth and biofilm formation and whether it may have synergistic killing effects ... More
LuxS mediates iron-dependent biofilm formation, competence, and fratricide in Streptococcus pneumoniae.
AuthorsTrappetti C, Potter AJ, Paton AW, Oggioni MR, Paton JC,
JournalInfect Immun
PubMed ID21875962
During infection, Streptococcus pneumoniae exists mainly in sessile biofilms rather than in planktonic form, except during sepsis. The capacity to form biofilms is believed to be important for nasopharyngeal colonization as well as disease pathogenesis, but relatively little is known about the regulation of this process. Here, we investigated the ... More
Cell-type-specific nuclei purification from whole animals for genome-wide expression and chromatin profiling.
An understanding of developmental processes requires knowledge of transcriptional and epigenetic landscapes at the level of tissues and ultimately individual cells. However, obtaining tissue- or cell-type-specific expression and chromatin profiles for animals has been challenging. Here we describe a method for purifying nuclei from specific cell types of animal models ... More
Essential role of ARID2 protein-containing SWI/SNF complex in tissue-specific gene expression.
AuthorsXu F, Flowers S, Moran E,
JournalJ Biol Chem
PubMed ID22184115
Unfolding of the gene expression program that converts precursor cells to their terminally differentiated counterparts is critically dependent on the nucleosome-remodeling activity of the mammalian SWI/SNF complex. The complex can be powered by either of two alternative ATPases, BRM or BRG1. BRG1 is critical for development and the activation of ... More
Defects in Glanzmann thrombasthenia and LAD-III (LAD-1/v) syndrome: the role of integrin ß1 and ß3 in platelet adhesion to collagen.
Authorsvan de Vijver E, De Cuyper IM, Gerrits AJ, Verhoeven AJ, Seeger K, Gutiérrez L, van den Berg TK, Kuijpers TW,
JournalBlood
PubMed ID22065596
Patients with Glanzmann thrombasthenia or Leukocyte Adhesion Deficiency-III syndrome (LAD-III or LAD-1/variant) present with increased bleeding tendency because of the lack or dysfunction of the fibrinogen receptor GPIIb/IIIa (integrin aIIbß3), respectively. Although the bleeding disorder is more severe in LAD-III patients, classic aggregometry or perfusion of Glanzmann or LAD-III platelets ... More
Inhibitory effect of LXR activation on cell proliferation and cell cycle progression through lipogenic activity.
AuthorsKim KH, Lee GY, Kim JI, Ham M, Won Lee J, Kim JB,
JournalJ Lipid Res
PubMed ID20847297
Liver X receptor (LXR), a sterol-activated nuclear hormone receptor, has been implicated in cholesterol and fatty acid homeostasis via regulation of reverse cholesterol transport and de novo fatty acid synthesis. LXR is also involved in immune responses, including anti-inflammatory action and T cell proliferation. In this study, we demonstrated that ... More
Glucose-based peritoneal dialysis solution suppresses adiponectin synthesis through oxidative stress in an experimental model of peritoneal dialysis.
AuthorsHuh JY, Seo EY, Lee HB, Ha H,
JournalPerit Dial Int
PubMed ID20798331
Accumulation of visceral fat is one of the major risk factors for the development of cardiovascular disease in peritoneal dialysis (PD) patients. Adiponectin, an adipokine commonly regarded as a negative indicator of metabolic disease, is reported to be downregulated in its gene level in end-stage renal disease patients. Since excessive ... More
Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.
AuthorsFernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,
JournalTissue Eng Part C Methods
PubMed ID30412046
cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More