Punicalagin Prevents Inflammation in LPS-Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway.
AuthorsCao Y, Chen J, Ren G, Zhang Y, Tan X, Yang L,
JournalNutrients
PubMed ID31731808
'Punicalagin, a hydrolysable tannin of pomegranate juice, exhibits multiple biological effects, including inhibiting production of pro-inflammatory cytokines in macrophages. Autophagy, an intracellular self-digestion process, has been recently shown to regulate inflammatory responses. In this study, we investigated the anti-inflammatory potential of punicalagin in lipopolysaccharide (LPS) induced RAW264.7 macrophages and uncovered ... More
In vitro assay for the efficacy assessment of AAV vectors expressing microdystrophin.
AuthorsDanilov KA, Vassilieva SG, Polikarpova AV, Starikova AV, Shmidt AA, Galkin II, Tsitrina AA, Egorova TV, Orlov SN, Kotelevtsev YV,
JournalExp Cell Res
PubMed ID32360435
'AAV-delivered microdystrophin genes hold great promise for Duchenne muscular dystrophy (DMD) treatment. It is anticipated that the optimization of engineered dystrophin genes will be required to increase the efficacy and reduce the immunogenicity of transgenic proteins. An in vitro system is required for the efficacy testing of genetically engineered dystrophin ... More
Co-regulation of the transcription controlling ATF2 phosphoswitch by JNK and p38.
AuthorsKirsch K, Zeke A, Toke O, Sok P, Sethi A, Sebo A, Kumar GS, Egri P, Póti ÁL, Gooley P, Peti W, Bento I, Alexa A, Reményi A,
JournalNat Commun
PubMed ID33188182
'Transcription factor phosphorylation at specific sites often activates gene expression, but how environmental cues quantitatively control transcription is not well-understood. Activating protein 1 transcription factors are phosphorylated by mitogen-activated protein kinases (MAPK) in their transactivation domains (TAD) at so-called phosphoswitches, which are a hallmark in response to growth factors, cytokines ... More
Escape from neutralizing antibodies by SARS-CoV-2 spike protein variants.
AuthorsWeisblum Y, Schmidt F, Zhang F, DaSilva J, Poston D, Lorenzi JC, Muecksch F, Rutkowska M, Hoffmann HH, Michailidis E, Gaebler C, Agudelo M, Cho A, Wang Z, Gazumyan A, Cipolla M, Luchsinger L, Hillyer CD, Caskey M, Robbiani DF, Rice CM, Nussenzweig MC, Hatziioannou T, Bieniasz PD,
JournalElife
PubMed ID33112236
'Neutralizing antibodies elicited by prior infection or vaccination are likely to be key for future protection of individuals and populations against SARS-CoV-2. Moreover, passively administered antibodies are among the most promising therapeutic and prophylactic anti-SARS-CoV-2 agents. However, the degree to which SARS-CoV-2 will adapt to evade neutralizing antibodies is unclear. ... More
Clinical utility of circulating tumor-associated cells to predict and monitor chemo-response in solid tumors.
AuthorsCrook T, Gaya A, Page R, Limaye S, Ranade A, Bhatt A, Patil S, Kumar P, Patil D, Akolkar D,
JournalCancer Chemother Pharmacol
PubMed ID33170321
'Selection of cytotoxic chemotherapy agents (CCA) based on pre-treatment evaluation of drug sensitivities is a desirable but unmet goal for personalized anticancer treatment strategies. Prior attempts to correlate in vitro Chemo-Response Profiles (CRP) of tumor explants or Circulating Tumor Cells (CTCs) with clinical outcomes have been largely unsuccessful. We present ... More
MicroRNA-103a Curtails the Stemness of Non-Small Cell Lung Cancer Cells by Binding OTUB1 via the Hippo Signaling Pathway.
AuthorsHu Z, Xiao D, Qiu T, Li J, Liu Z,
JournalTechnol Cancer Res Treat
PubMed ID33174524
'Although microRNA-103a (miR-103a) dysfunction has been implicated in various cancers, its relevance to non-small cell lung cancer (NSCLC) has not been clarified. This study was conducted to examine the molecular mechanism underlying the regulatory role of miR-103a in NSCLC. Kaplan-Meier analysis was carried out to assess the relationship between overall ... More
Imaging of multiple fluorescent proteins in canopies enables synthetic biology in plants.
AuthorsRigoulot SB, Schimel TM, Lee JH, Sears RG, Brabazon H, Layton JS, Li L, Meier KA, Poindexter MR, Schmid MJ, Seaberry EM, Brabazon JW, Madajian JA, Finander MJ, DiBenedetto J, Occhialini A, Lenaghan SC, Stewart CN,
JournalPlant Biotechnol J
PubMed ID33179383
'Reverse genetics approaches have revolutionized plant biology and agriculture. Phenomics has the prospect of bridging plant phenotypes with genes, including transgenes, to transform agricultural fields. Genetically encoded fluorescent proteins (FPs) have revolutionized plant biology paradigms in gene expression, protein trafficking and plant physiology. While the first instance of plant canopy ... More
The Serine Protease HTRA-1 Is a Biomarker for ROP and Mediates Retinal Neovascularization.
'Retinopathy of prematurity (ROP) is a blinding aberrancy of retinal vascular maturation in preterm infants. Despite delayed onset after preterm birth, representing a window for therapeutic intervention, we cannot prevent or cure ROP blindness. A natural form of ROP protection exists in the setting of early-onset maternal preeclampsia, though is ... More
A Public BCR Present in a Unique Dual-Receptor-Expressing Lymphocyte from Type 1 Diabetes Patients Encodes a Potent T Cell Autoantigen.
AuthorsAhmed R, Omidian Z, Giwa A, Cornwell B, Majety N, Bell DR, Lee S, Zhang H, Michels A, Desiderio S, Sadegh-Nasseri S, Rabb H, Gritsch S, Suva ML, Cahan P, Zhou R, Jie C, Donner T, Hamad ARA,
JournalCell
PubMed ID31150624
'T and B cells are the two known lineages of adaptive immune cells. Here, we describe a previously unknown lymphocyte that is a dual expresser (DE) of TCR and BCR and key lineage markers of both B and T cells. In type 1 diabetes (T1D), DEs are predominated by one clonotype ... More
Ascorbic Acid Promotes Functional Restoration after Spinal Cord Injury Partly by Epigenetic Modulation.
AuthorsHong JY, Davaa G, Yoo H, Hong K, Hyun JK,
JournalCells
PubMed ID32466098
'Axonal regeneration after spinal cord injury (SCI) is difficult to achieve, and no fundamental treatment can be applied in clinical settings. DNA methylation has been suggested to play a role in regeneration capacity and neuronal growth after SCI by controlling the expression of regeneration-associated genes (RAGs). The aim of this ... More
Comparative genomics of rice false smut fungi Ustilaginoidea virens Uv-Gvt strain from India reveals genetic diversity and phylogenetic divergence.
AuthorsPramesh D, Prasannakumar MK, Muniraju KM, Mahesh HB, Pushpa HD, Manjunatha C, Saddamhusen A, Chidanandappa E, Yadav MK, Kumara MK, Sharanabasav H, Rohith BS, Banerjee G, Das AJ,
Journal3 Biotech
PubMed ID32714737
'False smut disease of rice caused by Ustilaginoidea virens, is an emerging threat to rice cultivation worldwide due to its detrimental effects on grain yield and quality. False smut disease severity was 4.44?17.22% during a roving survey in Kharif 2016 in the four different rice ecosystems of Karnataka, India. Further, ... More
Expression profiles of miRNAs in giant cell tumor of bone showed miR-187-5p and miR-1323 can regulate biological functions through inhibiting FRS2.
AuthorsJin Y, Zhang J, Zhu H, Fan G, Zhou G,
JournalCancer Med
PubMed ID32154662
'Giant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. MicroRNAs are one type of small noncoding RNA, which can inhibit the translation of targeted mRNA through RNA-induced silencing complex. Microarray was conducted on three groups of ... More
Modular Polymer Antigens To Optimize Immunity.
AuthorsBennett NR, Jarvis CM, Alam MM, Zwick DB, Olson JM, Nguyen HV, Johnson JA, Cook ME, Kiessling LL,
JournalBiomacromolecules
PubMed ID31609600
Subunit vaccines can have excellent safety profiles, but their ability to give rise to robust immune responses is often compromised. For glycan-based vaccines, insufficient understanding of B and T cell epitope combinations that yield optimal immune activation hinders optimization. To determine which antigen features promote desired IgG responses, we synthesized ... More
Adenovirus VA RNAI Blocks ASC Oligomerization and Inhibits NLRP3 Inflammasome Activation.
AuthorsDarweesh M, Kamel W, Gavrilin MA, Akusjärvi G, Svensson C,
JournalFront Immunol
PubMed ID31849970
Virus infected immune cells can rapidly respond to the invader by activating the inflammasome and as a consequence release proinflammatory cytokines and eventually die by pyroptosis. In human adenovirus-5 (Ad5) infected THP-1 cells, inhibition of NLRP3 inflammasome activation was demonstrated by a decreased secretion of HMGB1 and matured forms of ... More
Ginsenoside Rb1 Induces Beta 3 Adrenergic Receptor-Dependent Lipolysis and Thermogenesis in 3T3-L1 Adipocytes and db/db Mice.
AuthorsLim S, Park J, Um JY,
JournalFront Pharmacol
PubMed ID31680950
Obesity is constantly rising into a major health threat worldwide. Activation of brown-like transdifferentiation of white adipocytes (browning) has been proposed as a promising molecular target for obesity treatment. In this study, we investigated the effect of ginsenoside Rb1 (Rb1), a saponin derived from Panax ginseng Meyer, on browning. We ... More
Repetitive elements as a transcriptomic marker of aging: Evidence in multiple datasets and models.
AuthorsLaRocca TJ, Cavalier AN, Wahl D,
JournalAging Cell
PubMed ID32500641
Transcriptomic markers of aging can be useful for studying age-related processes and diseases. However, noncoding repetitive element (RE) transcripts, which may play an important role in aging, are commonly overlooked in transcriptome studies-and their potential as a transcriptomic marker of aging has not been evaluated. Here, we used multiple RNA-seq ... More
The MARCH6-SQLE Axis Controls Endothelial Cholesterol Homeostasis and Angiogenic Sprouting.
AuthorsTan JME, van der Stoel MM, van den Berg M, van Loon NM, Moeton M, Scholl E, van der Wel NN, Kovacevic I, Hordijk PL, Loregger A, Huveneers S, Zelcer N,
JournalCell Rep
PubMed ID32755570
The endothelial monolayer forms a barrier between the lumen of blood vessels and the underlying tissues. Stable VE-cadherin-based adherens junctions are essential for maintaining this barrier, whereas their remodeling is required for angiogenesis in health and disease. Here, we position the ERAD-associated ubiquitin ligase MARCH6 as a determinant of angiogenic ... More
DLC1 is a direct target of activated YAP/TAZ that drives collective migration and sprouting angiogenesis.
Authorsvan der Stoel M, Schimmel L, Nawaz K, van Stalborch AM, de Haan A, Klaus-Bergmann A, Valent ET, Koenis DS, van Nieuw Amerongen GP, de Vries CJ, de Waard V, Gloerich M, van Buul JD, Huveneers S,
JournalJ Cell Sci
PubMed ID31964713
Endothelial YAP/TAZ (YAP is also known as YAP1, and TAZ as WWTR1) signaling is crucial for sprouting angiogenesis and vascular homeostasis. However, the underlying molecular mechanisms that explain how YAP/TAZ control the vasculature remain unclear. This study reveals that the focal adhesion protein deleted-in-liver-cancer 1 (DLC1) is a direct transcriptional ... More
Norepinephrine-Induced DNA Damage in Ovarian Cancer Cells.
AuthorsLamboy-Caraballo R, Ortiz-Sanchez C, Acevedo-Santiago A, Matta J, N A Monteiro A, N Armaiz-Pena G,
JournalInt J Mol Sci
PubMed ID32213975
Multiple studies have shown that psychological distress in epithelial ovarian cancer (EOC) patients is associated with worse quality of life and poor treatment adherence. This may influence chemotherapy response and prognosis. Moreover, although stress hormones can reduce cisplatin efficacy in EOC treatment, their effect on the integrity of DNA remains ... More
Genetic and Phenotypic Characterization of a Rabies Virus Strain Isolated from a Dog in Tokyo, Japan in the 1940s.
AuthorsTakahashi T, Inukai M, Sasaki M, Potratz M, Jarusombuti S, Fujii Y, Nishiyama S, Finke S, Yamada K, Sakai H, Sawa H, Nishizono A, Sugiyama M, Ito N,
JournalViruses
PubMed ID32825306
The rabies virus strain Komatsugawa (Koma), which was isolated from a dog in Tokyo in the 1940s before eradication of rabies in Japan in 1957, is known as the only existent Japanese field strain (street strain). Although this strain potentially provides a useful model to study rabies pathogenesis, little is ... More
Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions.
AuthorsBurt M, Bhattachaya R, Okafor AE, Musah S,
JournalJ Vis Exp
PubMed ID32716365
Kidney disease affects more than 10% of the global population and costs billions of dollars in federal expenditures. The most severe forms of kidney disease and eventual end-stage renal failure are often caused by the damage to the glomerular podocytes, which are the highly specialized epithelial cells that function together ... More
Triamcinolone-Gold Nanoparticles Repolarize Synoviocytes and Macrophages in an Inflamed Synovium.
AuthorsPark JY, Kwon S, Kim SH, Kang YJ, Khang D,
JournalACS Appl Mater Interfaces
PubMed ID32805872
Understanding the crosstalk between synoviocytes and macrophages is very important for the development of strategies to regulate inflammatory responses in an inflamed synovium. Simultaneous regulation of the pro- and anti-inflammatory responses of synoviocytes and macrophages (repolarization) is critical for the treatment of arthritis. Thus, the immune regulatory functions of an ... More
Bi-allelic Missense Pathogenic Variants in TRIP13 Cause Female Infertility Characterized by Oocyte Maturation Arrest.
AuthorsZhang Z, Li B, Fu J, Li R, Diao F, Li C, Chen B, Du J, Zhou Z, Mu J, Yan Z, Wu L, Liu S, Wang W, Zhao L, Dong J, He L, Liang X, Kuang Y, Sun X, Sang Q, Wang L,
JournalAm J Hum Genet
PubMed ID32473092
Normal oocyte meiosis is a prerequisite for successful human reproduction, and abnormalities in the process will result in infertility. In 2016, we identified mutations in TUBB8 as responsible for human oocyte meiotic arrest. However, the underlying genetic factors for most affected individuals remain unknown. TRIP13, encoding an AAA-ATPase, is a ... More
CD4 T-cell immune stimulation of HER2?+?breast cancer cells alters response to trastuzumab in vitro.
AuthorsSong PN, Mansur A, Dugger KJ, Davis TR, Howard G, Yankeelov TE, Sorace AG,
JournalCancer Cell Int
PubMed ID33292267
The HER2?+?tumor immune microenvironment is composed of macrophages, natural killer cells, and tumor infiltrating lymphocytes, which produce pro-inflammatory cytokines. Determining the effect of T-cells on HER2?+?cancer cells during therapy could guide immunogenic therapies that trigger antibody-dependent cellular cytotoxicity. This study utilized longitudinal in vitro time-resolved microscopy to measure T-cell influence ... More
The deubiquitinase STAMBP modulates cytokine secretion through the NLRP3 inflammasome.
The NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is a multimeric, cytoplasmic, protein complex that regulates maturation and secretion of interleukin (IL)-1ß, a potent pro-inflammatory cytokine. Critical to host defense against pathogens, IL-1ß amplifies early innate immune responses by activating transcription of numerous other cytokines and chemokines. Excessive ... More
Gut Bacterial Metabolite Urolithin A Decreases Actin Polymerization and Migration in Cancer Cells.
AuthorsAlauddin M, Okumura T, Rajaxavier J, Khozooei S, Pöschel S, Takeda S, Singh Y, Brucker SY, Wallwiener D, Koch A, Salker MS,
JournalMol Nutr Food Res
PubMed ID31976617
Urolithin A (UA) is a gut-derived bacterial metabolite from ellagic acid found in pomegranates, berries, and nuts can downregulate cell proliferation and migration. Cell proliferation and cell motility require actin reorganization, which is under control of ras-related C3 botulinum toxin substrate 1 (Rac1) and p21 protein-activated kinase 1 (PAK1). The ... More
Generation of two human control iPS cell lines (UCLi016-A and UCLi017-A) from healthy donors with no known ocular conditions.
AuthorsMéjécase C, Harding P, Sarkar H, Eintracht J, Lima Cunha D, Toualbi L, Moosajee M,
JournalStem Cell Res
PubMed ID33370878
Two human induced pluripotent stem cell (hiPSC) lines (UCLi016-A and UCLi017-A) were generated from fibroblast cells of 23- and 34-year-old healthy male donors with no known ocular conditions. Fibroblast cells were derived from skin biopsies and reprogrammed using integration free episomal reprogramming. The established iPSC lines were found to express ... More
D1 receptors in the anterior cingulate cortex modulate basal mechanical sensitivity threshold and glutamatergic synaptic transmission.
AuthorsDarvish-Ghane S, Quintana C, Beaulieu JM, Martin LJ,
JournalMol Brain
PubMed ID32891169
The release of dopamine (DA) into target brain areas is considered an essential event for the modulation of many physiological effects. While the anterior cingulate cortex (ACC) has been implicated in pain related behavioral processes, DA modulation of synaptic transmission within the ACC and pain related phenotypes remains unclear. Here we ... More
Functions of Exogenous RUNX2 in Giant Cell Tumor of Bone In Vitro.
AuthorsJin YH, Zhang J, Zhu H, Fan GT, Zhou GX,
JournalOrthop Surg
PubMed ID32154660
This research aimed to investigate the relative level of Runt-related transcription factor 2 (RUNX2) in giant cell tumor of bone (GCTB). Through the histopathological similarities between osteoporosis and GCTB, the biological functions of exogenous RUNXS were demonstrated in GCTB cell lines. This generated awareness of the molecular mechanism of the ... More
Analysis of lung stromal expression of the atypical chemokine receptor ACKR2 reveals unanticipated expression in murine blood endothelial cells.
AuthorsHansell CAH, Love S, Pingen M, Wilson GJ, MacLeod M, Graham GJ,
JournalEur J Immunol
PubMed ID32114694
Analysis of chemokine receptor, and atypical chemokine receptor, expression is frequently hampered by the lack of availability of high-quality antibodies and the species specificity of those that are available. We have previously described methodology utilizing Alexa-Fluor-labeled chemokine ligands as versatile reagents to detect receptor expression. Previously this has been limited ... More
Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton's jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis.
AuthorsShi Q, Sun B, Wang D, Zhu Y, Zhao X, Yang X, Zhang Y,
JournalStem Cell Res Ther
PubMed ID33261656
Accumulating evidence indicates that mesenchymal stem cells (MSCs) exert tissue repair effects and therapeutic angiogenesis through their noncoding RNAs (ncRNAs). Our previous studies showed that MSCs derived from Wharton's jelly (WJ-MSCs) can ameliorate damaged human endometrium by promoting angiogenesis. There is limited information on the functions and mechanism of ncRNAs ... More
miR-337-3p inhibits gastric tumor metastasis by targeting ARHGAP10.
AuthorsWang Z, Yao L, Li Y, Hao B, Wang M, Wang J, Gu W, Zhan H, Liu G, Wu Q,
JournalMol Med Rep
PubMed ID31789419
Several microRNAs (miRNAs) are known as regulatory molecules involved in gastric tumor metastasis. The expression of miR-337-3p was revealed to be downregulated in metastatic gastric tumor cells. Overexpression of miR-337-3p in gastric cancer cells resulted in the reduction of their invasive abilities. To characterize the functions of miR-337-3p, miR-337-3p was ... More