Amplified RNA synthesized from limited quantities of heterogeneous cDNA.
AuthorsVan Gelder RN, von Zastrow ME, Yool A, Dement WC, Barchas JD, Eberwine JH,
JournalProc Natl Acad Sci U S A
PubMed ID1689846
The heterogeneity of neural gene expression and the spatially limited expression of many low-abundance messenger RNAs in the brain has made cloning and analysis of such messages difficult. To generate amounts of nucleic acids sufficient for use in standard cloning strategies, we have devised a method for producing amplified heterogeneous ... More