Countess™ 3 FL Automated Cell Counter, 1 instrument - FAQs

View additional product information for Countess™ 3 FL Automated Cell Counter - FAQs (A49866, A50303, AMQAF2000, A49892, AMQAF2001, A50299, A49893)

8 product FAQs found

What cell counter do you offer that can count live and dead cells, measure cell viability, cell size, has high-resolution touch screen, and comes with reusable slides?

We suggest the Countess 3 and 3 FL Automated Cell Counters with starter packages; please see the product pages linked below:

Countess 3 FL Automated Cell Counter Starter Package + ABRC Extended Warranty (Cat. No. A50303)

Countess 3 FL Automated Cell Counter Starter Package + REX Extended Warranty (Cat. No. A50299)

Countess 3 FL Automated Cell Counter Starter Package (Cat. No. A49866)

Countess 3 Automated Cell Counter Starter Package + ABRC Extended Warranty (Cat. No. A50302)

Countess 3 Automated Cell Counter Starter Package + REX Extended Warranty (Cat. No. A50298)

Countess 3 Automated Cell Counter Starter Package (Cat. No A49865)

For more information about out Countess 3 Automated Cell Counters See the brochure linked below:

linkCountess 3 Automated Cell Counters: A counter that thinks like a cell biologist

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why are my cell counts twice as high when using Bright Field (BF) counting on the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000), compared to BF+GFP counting, when using the same sample?

The reason for this difference is that the bright field‐only counting assumes that the sample is diluted 1:1 and corrects the result automatically by multiplying it with 2. When you count in the GFP-mode the instrument assumes trypan blue was not used and thus sample not diluted. The total cell concentration displayed after a fluorescent count does not take any dilution into account. Therefore, the results reflect the actual cell concentration in the sample slide, which must be multiplied by any dilution factor present to calculate the original cell concentration.

Find additional tips, troubleshooting help, and resources within our Cell Counting Support Center.

What is the alternative to TrypanBlue for using it with the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000?

The suitable alternative would be Erythrosin B (Cat. No. 409451000, A14180.36, 189460250 and J62619.22).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are there any recommended parameter settings on the Countess 3/3FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) for specific cell lines?

No. The Countess 3/3 FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) parameter settings, especially brightness, can vary between instruments due to slight differences in lamp output and other factors.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can we do with the FCS file generated by the Countess 3/3 FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000)?

The Countess 3/3FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) FCS files can be uploaded to most flow cytometry software that can accept FCS files, such as FlowJo or the Attune NxT software. Using the flow cytometry software, you may see the distribution of all cells and adjust/fine-tune parameter settings for only the cells of interest or analyze outliers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can we use any EVOS Light Cube with the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000)?

With the exception of EVOS Light Cube, White (Cat no. AMEP4672) any of the EVOS Light Cubes for fluorescence detection can be used on the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I count nuclei with the Countess 3 Automated Cell Counter (Cat. No. AMQAX2000) or Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000)?

Yes, with software update version 1.3.1324.552, nuclei can be counted using the Countess 3 FL Automated Cell Counter. We recommend performing FL-based counting. Please refer to this application note for detailed instructions.

Find additional tips, troubleshooting help, and resources within our Cell Counting Support Center.

What is the optimal cell size and density for accurate cell counting with the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000)?

Cells ranging in size from 5 µm to 60 µm and cell suspension density ranging from 1 x 10E5 to 10E6 cells/mL are optimal for counting with the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000).