Countess™ 3 Automated Cell Counter, 1 instrument - FAQs

View additional product information for Countess™ 3 Automated Cell Counter - FAQs (A49865, A50302, AMQAX2000, A49891, A50298, A49862)

7 product FAQs found

Why am I getting a much higher bead count on the Countess 3 Standard Slide (Cat. No. A51876)?

When counting cells that have been mixed with Trypan Blue, the Countess accounts for this 1:1 dilution and multiplies the count by 2.
When using the Countess 3 Standard Slide, the Trypan Blue correction box needs to be unchecked, since a 1:1 dilution was not made with Trypan Blue.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What cell counter do you offer that can count live and dead cells, measure cell viability, cell size, has high-resolution touch screen, and comes with reusable slides?

We suggest the Countess 3 and 3 FL Automated Cell Counters with starter packages; please see the product pages linked below:

Countess 3 FL Automated Cell Counter Starter Package + ABRC Extended Warranty (Cat. No. A50303)

Countess 3 FL Automated Cell Counter Starter Package + REX Extended Warranty (Cat. No. A50299)

Countess 3 FL Automated Cell Counter Starter Package (Cat. No. A49866)

Countess 3 Automated Cell Counter Starter Package + ABRC Extended Warranty (Cat. No. A50302)

Countess 3 Automated Cell Counter Starter Package + REX Extended Warranty (Cat. No. A50298)

Countess 3 Automated Cell Counter Starter Package (Cat. No A49865)

For more information about out Countess 3 Automated Cell Counters See the brochure linked below:

linkCountess 3 Automated Cell Counters: A counter that thinks like a cell biologist

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are there any recommended parameter settings on the Countess 3/3FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) for specific cell lines?

No. The Countess 3/3 FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) parameter settings, especially brightness, can vary between instruments due to slight differences in lamp output and other factors.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can we do with the FCS file generated by the Countess 3/3 FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000)?

The Countess 3/3FL Automated Cell Counter (Cat. Nos. AMQAF2000, AMQAX2000) FCS files can be uploaded to most flow cytometry software that can accept FCS files, such as FlowJo or the Attune NxT software. Using the flow cytometry software, you may see the distribution of all cells and adjust/fine-tune parameter settings for only the cells of interest or analyze outliers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can we use any EVOS Light Cube with the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000)?

With the exception of EVOS Light Cube, White (Cat no. AMEP4672) any of the EVOS Light Cubes for fluorescence detection can be used on the Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use crystal violet with the Countess 3 (Cat. No. AMQAX2000) and 3 FL Automated Cell Counter (Cat. No. AMQAF2000)?

Yes. Crystal violet is compatible with Countess 3 (Cat. No. AMQAX2000) and 3 FL Automated Cell Counter (AMQAF2000) under brightfield mode detection.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

Can I count nuclei with the Countess 3 Automated Cell Counter (Cat. No. AMQAX2000) or Countess 3 FL Automated Cell Counter (Cat. No. AMQAF2000)?

Yes, with software update version 1.3.1324.552, nuclei can be counted using the Countess 3 FL Automated Cell Counter. We recommend performing FL-based counting. Please refer to this application note for detailed instructions.

Find additional tips, troubleshooting help, and resources within our Cell Counting Support Center.