20X Bolt™ MOPS SDS 电泳缓冲液
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20X Bolt™ MOPS SDS 电泳缓冲液
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20X Bolt™ MOPS SDS 电泳缓冲液

Bolt MOPS SDS 电泳缓冲液 (20X) 专为在 Bolt Bis-Tris Plus 预制胶中进行蛋白电泳而配制了解更多信息
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货号数量
B0001500 mL
B0001025 L
货号 B0001
价格(CNY)
1,110.00
Each
添加至购物车
数量:
500 mL
价格(CNY)
1,110.00
Each
添加至购物车
Bolt MOPS SDS 电泳缓冲液 (20X) 专为在 Bolt Bis-Tris Plus 预制胶中进行蛋白电泳而配制。推荐用于分离中等至高分子量的蛋白质。

使用合适的缓冲液优化蛋白质分离
Bolt MES SDS 电泳缓冲液和 Bolt MOPS SDS 电泳缓冲液均可搭配 Bolt Bis-Tris Plus 预制胶使用。MES 具有更低的 pKa,使得 MES SDS 电泳缓冲液的速度比 MOPS SDS 电泳缓冲液更快。不同缓冲液的离子迁移效应的差异会影响积层,进而导致蛋白质分离范围差异。使用 MOPS 缓冲液可使蛋白质电泳速度比使用 MES 缓冲液时慢

在 Bolt Bis-Tris Plus 预制胶上使用 MES 和 MOPS 比较蛋白质的迁移模式

请参阅所有可用于 SDS-PAGE 的缓冲液和试剂

仅供科研使用。不可用于诊断程序。
规格
化学名称或材料电泳缓冲液
建议的储存条件MOPS SDS 电泳缓冲液 (20X)。

室温储存。

如果储存得当,保质期为 6 个月。
最大浓度20 X
适用于(设备)Bolt™
物理形态Liquid
产品线Bolt™
数量500 mL
Unit SizeEach

常见问题解答 (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.