A new homogeneous high-throughput screening assay for profiling compound activity on the human ether-a-go-go-related gene channel.
AuthorsTitus SA, Beacham D, Shahane SA, Southall N, Xia M, Huang R, Hooten E, Zhao Y, Shou L, Austin CP, Zheng W,
JournalAnal Biochem
PubMed ID19583963
Long QT syndrome, either inherited or acquired from drug treatments, can result in ventricular arrhythmia (torsade de pointes) and sudden death. Human ether-a-go-go-related gene (hERG) channel inhibition by drugs is now recognized as a common reason for the acquired form of long QT syndrome. It has been reported that more ... More
Cell-based potassium ion channel screening using the FluxOR assay.
AuthorsBeacham DW, Blackmer T, O' Grady M, Hanson GT,
JournalJ Biomol Screen
PubMed ID20208034
FluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel activity, the FluxOR Potassium Ion Channel Assay is based on the activation of a novel fluorescent dye. This indicator reports channel activity with a ... More