BacMam 增强剂试剂盒
BacMam 增强剂试剂盒
Invitrogen™

BacMam 增强剂试剂盒

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells. To use, simply add the BacMam enhancer了解更多信息
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货号数量
B101071 kit
货号 B10107
价格(CNY)
1,825.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
1,825.00
Each
添加至购物车
The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.

To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells for 1.5–2 hours at 37°C and 5% CO2 and replace with fresh medium. Different cell types vary in their response to the BacMam enhancer and for best results the amount and treatment time will need to be optimized. Once prepared, the enhancer solution is stable to multiple freeze⁄thaw cycles following the recommended storage conditions. If desired, the solution can be divided into aliquots following reconstitution.
仅供科研使用。不可用于诊断程序。
规格
抑制剂组蛋白脱乙酰酶抑制剂
产品线分子探针™
数量1 kit
运输条件室温
比抑制或靶标组蛋白脱乙酰酶
标签类型荧光蛋白
产品类型BacMam 增强剂试剂盒
亚细胞定位Actin, Endoplasmic Reticulum, Endosomes, Golgi, Mitochondria
Unit SizeEach
内容与储存
每个试剂盒含有用于处理 10 X 96 微孔板所需的足够材料。在 2—6°C 下储存,切勿冷冻。根据说明储存时,产品可保持稳定 6 个月。

常见问题解答 (FAQ)

我使用你们的CellLight BacMam 试剂对我的细胞器进行荧光蛋白标记,但是标记效果不佳。我该如何加以改善?

首先,确定你们的细胞是哺乳动物且非血源性细胞。如果细胞转导效率不佳(例如只有少数细胞被标记或是标记荧光很暗),先试着增加每个细胞的微粒数。接下来试着使用BacMam Enhancer Solution。但是,如果你们的细胞显示标记而标记定位似乎太过普遍或是无处不在,那么你们应该降低每个细胞的微粒数。该手册给出了计算每个细胞的微粒数的指南和公式。

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using your CellLight BacMam reagents for fluorescent protein-labeling of my cell organelles, but they aren't labeling well. How can I improve it?

First, make sure that your cells are mammalian and non-blood derived. If the cells have low transduction efficiency (i.e., only a few cells are labeling or are labeling only dimly), first try increasing the particle number per cell. The next thing to try is to use the BacMam Enhancer Solution. If, however, your cells show labeling, but the localization seems to be too generalized or ubiquitous within the cell, then you should reduce the particle number per cell. The manual gives a guideline and formula for calculating particle number per cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.