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引用和文献 (8)
Invitrogen™
生物素炔烃（PEG4 羧酰胺-炔丙基生物素）
半抗原（生物素叠氮化物）通过铜催化点击反应与末端发生反应。随后可通过链霉素亲和素、亲和素或中性抗生物素蛋白®生物素结合蛋白检测生物素。了解更多信息
| 货号 | 数量 |
|---|---|
| B10185 | 1 mg |
货号 B10185
价格（CNY)
7,427.00
Each
数量:
1 mg
价格（CNY)
7,427.00
Each
半抗原（生物素叠氮化物）通过铜催化点击反应与末端发生反应。随后可通过链霉素亲和素、亲和素或中性抗生物素蛋白®生物素结合蛋白检测生物素。
仅供科研使用。不可用于诊断程序。
规格
化学反应性叠氮化物
产品规格实心
标签或染料生物素
分子量528.66 Da
产品类型生物素炔烃
数量1 mg
反应基团炔烃
反应一部分炔烃
运输条件室温
溶解度DMSO (二甲亚砜)
颜色生物素
标签类型生物素和其他半抗原
产品线Molecular Probes
Unit SizeEach
内容与储存
在 ≤-20°C 下储存，并保持干燥。
引用和文献 (8)
引用和文献
Abstract
Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives.
Journal:Chem Res Toxicol
PubMed ID:18232660
'Polyunsaturated fatty acids (PUFA) are primary targets of free radical damage during oxidative stress. Diffusible electrophilic alpha,beta-unsaturated aldehydes, such as 4-hydroxynonenal (HNE), have been shown to modify proteins that mediate cell signaling (e.g., IKK and Keap1) and alter gene expression pathways responsible for inducing antioxidant genes, heat shock proteins, and
Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
Journal:J Am Chem Soc
PubMed ID:18683930
'We report an advanced chemoenzymatic labeling strategy for direct fluorescence detection of O-GlcNAc proteins in gels that facilitates proteomic studies and greatly extend the reach of existing technologies. These new tools also enable the expression and dynamics of O-GlcNAc modifications to be monitored by imaging in cells and tissues.
Comparative methods for analysis of protein covalent modification by electrophilic quinoids formed from xenobiotics.
Journal:Bioconjug Chem
PubMed ID:19301905
'Conjugation of biotin and fluorophore tags is useful for assaying covalent protein modification. Oxidative bioactivation of selective estrogen receptor modulators (SERMs) yields reactive quinoid electrophiles that covalently modify proteins, and bioactivation is associated with carcinogenic and chemopreventive effects. Identification of the protein targets of electrophilic metabolites is of general importance
HACE1 reduces oxidative stress and mutant Huntingtin toxicity by promoting the NRF2 response.
Journal:
PubMed ID:24516159
Oxidative stress plays a key role in late onset diseases including cancer and neurodegenerative diseases such as Huntington disease. Therefore, uncovering regulators of the antioxidant stress responses is important for understanding the course of these diseases. Indeed, the nuclear factor erythroid 2-related factor 2 (NRF2), a master regulator of the
O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation.
Journal:J Biol Chem
PubMed ID:19478079
CCAAT enhancer-binding protein (C/EBP)beta is a basic leucine zipper transcription factor family member, and can be phosphorylated, acetylated, and sumoylated. C/EBPbeta undergoes sequential phosphorylation during 3T3-L1 adipocyte differentiation. Phosphorylation on Thr(188) by MAPK or cyclin A/cdk2 primes the phosphorylations on Ser(184)/Thr(179) by GSK3beta, and these phosphorylations are required for the