用于微孔板测定的 BackDrop™ 背景抑制剂

大多数培养基都含有酚红，会淬灭可见光波长范围的荧光染料。大多数培养基还含有自发荧光成分——如核黄素——会降低信噪比。我们提供的FluoroBrite DMEM和基于HEPES的活细胞成像溶液已针对荧光成像应用进行了优化。此外，我们还提供多种不含酚红的培养基。但是，如果这些产品都不是您的试验的合理选择，为此我们还提供BackDrop 背景抑制剂 ReadyProbes 试剂，将其加入可淬灭培养基的自发荧光。

There are a number of reagents used to quench fluorescence on the outside of live cells or in the media (background fluorescence). Our Backdrop Background Suppressor reagents are a great option to quench blue, green, and red fluorescent dyes, various media components and fluorescent proteins. Another commonly used reagent is the cell impermeant colorimetric dye, Trypan blue.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. The BackDrop dyes will quench any fluorescence of a given wavelength in the extracellular media or buffer, but not inside live cells. It will not quench autofluorescence inside live cells unless the dye is engulfed.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Yes. The following components are sold separately:
Component B: PowerLoad Concentrate (Cat. No. P10020)
Component C: BackDrop Background Suppressor for Microplate Assays (Cat. No. B10512)
Component D: Probenecid, Water Soluble (Cat. No. P36400)

We have not tested this. The BackDrop Background Suppressor reagent is cell impermeant, but if the quenching is caused by a FRET interaction and, if the distance between the BackDrop Background Suppressor outside of the cell is within the Ro distance for FRET with the fluorescent dye in the inner leaflet of the membrane, quenching may still occur.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.