Detection and quantitation of hexa-histidine-tagged recombinant proteins on western blots and by a surface plasmon resonance biosensor technique.
AuthorsO'Shannessy DJ, O'Donnell KC, Martin J, Brigham-Burke M
JournalAnal Biochem
PubMed ID8533880
'The use of short peptide affinity tag sequences has become commonplace for the expression and purification of recombinant proteins. Many of these tags are antibody epitopes and detection of tagged proteins via Western blots is straightforward. However, the most common affinity tag used at present for the expression of recombinant ... More
Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors.
Authorsvan Bueren AL, Higgins M, Wang D, Burke RD, Boraston AB
JournalNat Struct Mol Biol
PubMed ID17187076
The ability of pathogenic bacteria to recognize host glycans is often essential to their virulence. Here we report structure-function studies of previously uncharacterized glycogen-binding modules in the surface-anchored pullulanases from Streptococcus pneumoniae (SpuA) and Streptococcus pyogenes (PulA). Multivalent binding to glycogen leads to a strong interaction with alveolar type II ... More
Single-step synthesis and characterization of biotinylated nitrilotriacetic acid, a unique reagent for the detection of histidine-tagged proteins immobilized on nitrocellulose.
AuthorsMcMahan SA, Burgess RR
JournalAnal Biochem
PubMed ID8619473
Using a one-step reaction, a bifunctional compound was synthesized for detecting histidine-tagged proteins immobilized on nitrocellulose. This compound has a biotin as one functional group and a nitrilotriacetic acid as the other. The nitrilotriacetic acid is used to chelate a Ni(II) ion at four of its six coordination sites. The ... More